浊点萃取-气相色谱法测定槲寄生中20种农药残留  

Determination of twenty pesticide residues in Viscum coloratum by gas chromatography using cloud-point extraction

作　　者：张舒婷[1] 陈晓辉[1] 于治国[1] 沈旭[1] 勾萌[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中国中药杂志》2009年第20期2577-2580,共4页China Journal of Chinese Materia Medica

摘　　要：目的:建立浊点萃取-气相色谱法测定槲寄生中有机氯和拟除虫菊酯类农药残留量的方法。方法:选用聚乙二醇辛基苯基醚(Triton X-100)作为提取剂,采用气相色谱法,Hp-5弹性石英毛细管色谱柱分离,电子捕获检测器进行检测,外标法计算含量。结果:有机氯和拟除虫菊酯分别在5～500,10～1000μg·L-1呈良好线性关系,相关系数均大于0.9978;检测限(LOD)1.5～7.5μg·kg-1;平均回收率为74.15%～111.6%,RSD4.0%～9.1%。结论:本方法简便、快速,适用于槲寄生中的农药多残留的测定。Objective：To establish a gas chromatography method for simultaneous determination of organochlorine and pyrethroid pesticide residues in Viscum coloratum by cloud-point extraction （CPE）. Method：Pesticides were extracted with the non-ionic surfactant Triton X-100. The apparatus was gas chromatography with electron capture detector and the separation was performed on an Hp-5 column. The pesticide residues were calculated by external standard method. Result：Good linear relation was obtained over the range of 5-500 μg·L-1 for organochlorine and 10-1 000 μg·L^-1 for pyrethroid. The limits of detection was 1.5-7.5 μg·kg^-1. The average recoveries of organochlorine and pyrethroid were 74.15%-111.6% with corresponding RSD of 4.0%-9.1%. Conclusion：The sample and rapid method was applied to pesticide residues determination.

关 键 词：浊点萃取法 聚乙二醇辛基苯基醚 气相色谱法 有机氯 拟除虫菊酯 槲寄生 

分 类 号：S481.8[农业科学—农药学]