钙网蛋白在不同预处理心肌细胞缺血再灌注的保护作用  

The role of calreticulin in three different kinds of preconditioning against myocardial ischemia/ reperfnsion injury in rats

作　　者：陈光献[1] 唐白云[1] 吴钟凯[1] 梁孟亚[1] 刘海[1] 

机构地区：[1]中山大学附属第一医院心脏外科,广州510080

出　　处：《中华实验外科杂志》2009年第11期1416-1419,共4页Chinese Journal of Experimental Surgery

基　　金：资助项目：国家杰出青年科学基金资助项目（30525020）;教育部博士点基金资助项目（20050558050）;人事部留学人员项目（粤人发2006-11）;广东省省科技厅国际合作项目（2007B050200024）;广东省卫生厅基金资助项目（B2007038,A2008173）

摘　　要：目的观察3种不同预处理对在体缺血再灌注心肌的保护作用，探讨钙网蛋白（CRT）在预处理心肌细胞缺血再灌注损伤的作用。方法将30只成年SD大鼠随机分成5组（n=6），分别为缺血再灌注组、缺血预处理组、腺苷预处理组、远程预处理组和假手术组。建立大鼠在体缺血再灌注损伤模型，观察各组缺血再灌注前后心功能变化，并检测再灌注末血清肌钙蛋白T（cTnT）、丙二醛（MDA）、超氧化物歧化酶（SOD）的变化以及心肌组织CRT的表达。结果缺血预处理组、远程预处理组与缺血再灌注组比较（±dp／dt max）有明显提高（P〈0．05）。缺血预处理组、腺苷预处理组、远程预处理组cTnT、MDA值均低于缺血再灌注组，SOD值高于缺血再灌注组（P〈0．05）；腺苷预处理组SOD值高于缺血预处理组和远程预处理组，cTnT值则低于后2组（P〈0．05）；缺血预处理组、腺苷预处理组、远程组与缺血再灌注组比较，CRT表达灰度值均显著降低（P〈0．05）。结论腺苷预处理、远程预处理均可以模拟缺血预处理的心肌保护作用；预处理可能通过下调钙网蛋白高表达减轻在体大鼠心肌细胞缺血再灌注损伤。Objective To evaluate the role of calreticulin （CRT） in myocardial protection of three different kinds of preconditioning against myocardial ischemia/reperfusion injury. Methods Thirty SD rats were randomly divided into 5 groups：isehemia reperfusion group （IR） , isehemia preconditioning group （ IP）, adenosine preconditioning group （AP） , remote preconditioning group （ RP）, and sham-operation group （blank control）. The ischemia/reperfusion model was established in vivo. Hemodynamic changes of heart function were observed, and serum cardiac Troponin T （cTnT）, superoxide dismutase （SOD）, malondialdehyde （MDA） and the expression of CRT in myoeardium were detected. Results ± dp/dt max, serum cTnT and MDA, and the expression of CRT in RP, AP and IP groups were siguificantly lower than those in IR group, also SOD level in these three groups was higher than in IR group （ P 〈 0.05 ）. AP group had a higher SOD level venous IP and RP groups （ P 〈 0.05 ） , also eTnT in AP group was lower than in IP and RP groups （ P 〈 0.05 ）. Conclusion Remote preconditioning may mimic the protective effect of ischemie preconditioning. Remote preconditioning attenuated myocardial isehemia/reperfusion injury in vivo possibly through down-regulating the CRT expression in rats.

关 键 词：心肌细胞 缺血预处理 腺苷 再灌注损伤 钙网蛋白 

分 类 号：R285.5[医药卫生—中药学]