土壤微生物DNA提取方法的研究  

A study on extraction methods of microbial DNA from soils

作　　者：陈邦[1,2] 范代娣[1] 王琰[3] 

机构地区：[1]西北大学化工学院,陕西西安710069 [2]西北大学合成与天然功能分子化学教育部重点实验室/化学与材料科学学院,陕西西安710069 [3]陕西省微生物研究所,陕西西安710043

出　　处：《西北大学学报（自然科学版）》2009年第5期785-788,共4页Journal of Northwest University（Natural Science Edition）

基　　金：国家高技术发展计划基金资助项目(2006AA02Z246)

摘　　要：目的研究土壤微生物DNA的提取方法。方法选用3种常见有效的土壤微生物DNA提取方法与本实验设计的土壤微生物DNA提取方法对麦田土壤微生物DNA进行提取比较。结果4种方法均能从麦田土壤中提取到片段长度大于22kb的DNA片段,不同方法提取的DNA浓度有一定的差异。提取得到的总DNA不需要纯化就可以用于PCR扩增,使用细菌16S rDNA的通用引物可以扩增得到相应的片段。结论该法操作简便、提取快速、DNA纯度和得率较高。Aim To report methods of DNA extraction from soil microorganism. Methods Preparing the commonused and effective methods of DNA extraction with the designed method of DNA extraction from cornfield microorganism. Results All the four methods can obtain DNA fragments that are longer than 22kb from the cornfield microorganism. However, the quality of extraction DNA are different to some extent. The extraction DNA without purification could be amplified, and the relevant fragments by PCR with 16S rDNA universal primer could be acquired. Conclusion The designed method of DNA extraction is easy operation, quickly extraction and the acquired DNA is high both in purity and quality.

关 键 词：土壤 DNA 提取方法 

分 类 号：Q939.1[生物学—微生物学]