好好芭水通道蛋白ScPIP1的功能验证与分析  

Over-expression of ScPIP1 and increase in stress resistance in tobacco

作　　者：白瑞雪[1] 张根发[1] 

机构地区：[1]北京师范大学生命科学学院,北京100875

出　　处：《西北大学学报（自然科学版）》2009年第5期796-800,共5页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(30670203)

摘　　要：目的对转ScPIP1基因烟草植株进行研究以检测ScPIP1的功能及转基因植株的抗逆能力。方法使用PEG-4000对材料进行人工模拟干旱胁迫;使用NaC l对材料进行盐胁迫;使用显微镜对材料叶片气孔形态及指标进行测定。材料的萌发及生长指标使用标尺测量。对所获数据进行统计学分析。结果Pt2的气孔密度是WT和Pt5的2倍,气孔开度则是WT大于Pt5,Pt5大于Pt2。在不同浓度PEG-4000和NaC l胁迫条件下,Pt2转基因植株的萌发率均大于WT和Pt5。且在胁迫下生长过程中,Pt2的根长与株高均大于WT和Pt5。结论ScPIP1参与了植物对非生物逆境的应答,并且Pt2是有较高抗逆能力的转基因系。Aim To make a study on tobaco and transgenic tobacco plants for delecting the fuctions of ScPIP1 and the stress resistant ability of ScPIP1 transgenic plants. Methods Using PEG-4000 for artificial simulation of drought stress; using NaC1 for salt stress. Using a microscope on the material morphology and stomatal index were determined. The germination and growth parameters of materials were measured using the ruler. All the data obtained were analyzed statistically. Results The stoma density of transgenic tobacco Pt2 was 2-fold higher than WT and PtS. The stomatal aperture of Pt5 was higher than Pt2, but lower than WT. Germinations of Pt2 seeds at PEG4000 and NaC1 concentration of different concentration were greater than wild-type and Pt5 seeds. When exposed to drought and salt stress, roots of Pt2 plants were longer and height was higher than that observed in WT and Pt5 plants. Conclusion These results are consistent with the notion that ScPIP1 was involved in response to abiotic stresses, and Pt2 was the line imoroved resistance of abiotic stresses.

关 键 词：好好芭 PIP1 转基因烟草 非生物逆境 

分 类 号：S565.901[农业科学—作物学]