iNOS在大鼠性成熟期睾丸组织的表达变化及意义  

The expression and significance of the iNOS in the sexual-maturity SD rat's testis

作　　者：任亚萍[1] 孙莉[1] 毛庭枝[1] 邵晓云[1] 陈筠[1] 农林琳[1] 

机构地区：[1]桂林医学院组胚教研室,广西桂林541004

出　　处：《华夏医学》2009年第5期841-843,共3页Acta Medicinae Sinica

摘　　要：目的:探讨诱导型一氧化氮合酶(iNOS)在雄性SD大鼠性成熟期睾丸中的表达变化情况及意义。方法:选取出生后5周、7周和10周的雄性SD大鼠各6只,左侧睾丸行石蜡切片,运用免疫组化ABC法观察iNOS在雄性SD大鼠性成熟期睾丸中的表达变化情况。结果:1出生后5周,iNOS睾丸间质细胞阴性表达。2出生后7周,绝大部分生精小管断面有精子发生,iNOS睾丸间质细胞弱阳性表达,精母细胞阳性表达。3出生后10周,iNOS睾丸间质细胞强阳性表达,精母细胞阳性表达。结论:在雄性SD大鼠的睾丸间质细胞中,随着性成熟发育iNOS的表达渐强。Objective： To explore the expression and significance of the inducible nitric oxide synthase （iNOS） in the sexual-maturity male SD rat＇s testis. Methods： Eighteen male SD rats born for 5 weeks, 7 weeks and 10 weeks were selected with each 6 rats. Paraffin sections of the left testis were made and the expression of the iNOS in the testis of male SD rat was observed by immunohistochemical ABC method. Results ： iNOS was expressed in Leydig celia of the testis, and the positive degree were（-）, （＋＋） and （＋＋＋） respectively in the 5-w, 7-w and 10-w groups. iNOS was also expressed in some spermatocytes. Conclusion： With the development process of Sexual maturation, positive expression of iNOS shows the trend of increasing in the Leydig cells of sexual-maturity male SD rat.

关 键 词：一氧化氮合酶 睾丸 大鼠 性成熟 

分 类 号：Q959.837[生物学—动物学] Q954.6