金鸡菊(Coreopsis drummondii)的抗TMV活性物质  

Anti-TMV Active Substances from Coreopsis drummondii

作　　者：陈启建[1] 欧阳明安[1] 吴祖建[1] 谢联辉[1] 林奇英[1] 

机构地区：[1]福建农林大学植物病毒研究所,福州350002

出　　处：《应用与环境生物学报》2009年第5期621-625,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.30770089);福建省教育厅项目(No.2006F5017)资助~~

摘　　要：采用活性跟踪法从金鸡菊根中分离获得抗病毒活性物质,经质谱和核磁共振分析,鉴定该物质为1-苯基-1,3,5-三庚炔.采用半叶枯斑法、叶圆盘法测定了该物质对烟草花叶病毒的抑制效果,结果表明,0.2mg/mL的该化合物对TMV表现出较好的体外抑制侵染和增殖活性,其对TMV侵染和复制的抑制率分别为73.5%和84.3%.实时荧光定量PCR测定结果表明,该化合物对TMV外壳蛋白基因的表达有明显的抑制作用,0.2mg/mL的该化合物对TMV外壳蛋白基因表达的抑制率为79.8%.Production of plant secondary metabolites as a rich source of antimicrobial agents has been extensively applied in Chinese traditional medicine to treat viral diseases. Therefore, direct selection of antiphytoviral compounds from plants can be used to identify new potent antiviral agents. To screen antiviral compounds, tobacco mosaic virus （TMV） was chosen as a model target and the extracts with anti-TMV activity from 108 species of plants were analyzed. Among them, a extract from Coreopsis drummondii, predominantly suppressing the infection and replication of TMV was selected, and a constituent with anti-virus activity was isolated from it by bioassay. The compound was identified as 1-phenyl-1,3,5-triheptalkyne using MS and NMR. Its activity against TMV was investigated by local lesion and leaf discs assay. The results indicated that the compound showed a significant inhibitory activity against TMV in vitro, with 73.5% inhibitory rate against the infection of TMV and 84.3% against replication of TMV at a concentration of 0.2 mg/mL, while its inhibitory effect on TMV-CP gene expression in tobacco leaf discs was examined by real-time quantitative PCR, which showed that the compound obviously suppressed the expression of TMV-CP gene, with 79.8% inhibitory rate at the concentration of 0.2 mg/mL. Taken together, the anti-TMV activity of the compound contributes itself as a new potential candidate for development of antiviral drugs. Fie 6. Tab 1. Ref 19

关 键 词：金鸡菊 抗TMV活性 1-苯基-1 3 5-三庚炔 活性物质分离 

分 类 号：S482.2[农业科学—农药学]