超级黑糯玉米花药单倍体诱导培养基的筛选  

Screening Anther Culture Medium of Super Black Waxy Corn

作　　者：朱艳芳[1,2] 薛建平[1,2] 蔡士兵[3] 王海生[1] 

机构地区：[1]淮北煤炭师范学院生命科学学院,安徽淮北235000 [2]资源植物生物学安徽省重点实验室,安徽淮北235000 [3]安徽省濉溪县农业科学研究所,安徽濉溪235100

出　　处：《玉米科学》2009年第5期65-67,共3页Journal of Maize Sciences

基　　金：安徽高校省级自然科学研究重点项目(KJ2008A012)

摘　　要：通过多因素正交实验发现,基本培养基种类和激素配比是影响超级黑糯玉米花药培养的重要因素,并确定N6和正14两种基本培养基较适合该类玉米花培诱导。进一步优化培养基的研究表明,两种培养基对该类玉米花药诱导效果最好。玉米花药培养对基因型依赖很大,且不同基因型的玉米花药诱导需要的基本培养基也不同。超级黑糯玉米花培较适合的培养基是N6和正14的液体和半固体两种培养基。Orthogonal test design was used to optimize the anther culture medium of super black waxy corn. The results showed that the induction frequency was highly dependent on genotype, the basic medium, the kind of hormone, and on their interactions. N6 and Zheng14 were better inducing basic media. Further researches showed that the two types of medium were the best drug-induced to the cornflakes. The conclusion was that different genotypes needed different basic media. So screening the optimal basic medium was the first of anther culture. And the optimal media of super black waxy corn were the liquid and the semisolid of N6 and Zheng14.

关 键 词：超级黑糯玉米 花药培养 单倍体 培养基 

分 类 号：S513.035.2[农业科学—作物学]