关白附中多糖含量的测定  

Determination of Polysaccharide Content in Aconitum coreanum

作　　者：冯凯[1] 佟海滨[1] 田丹[1] 刘扬[1] 初晓丹[1] 孙新[1] 

机构地区：[1]北华大学生命科学研究中心

出　　处：《北华大学学报（自然科学版）》2009年第5期436-438,共3页Journal of Beihua University（Natural Science）

基　　金：吉林省科技厅基金项目(20080906)

摘　　要：目的采用水提醇沉法提取关白附粗多糖.方法经反复冻融、酶法与Sevag法联合脱蛋白后测定关白附中多糖质量分数.结果多糖经苯酚-硫酸显色后在490 nm处用紫外可见分光光度计进行比色测定,测定结果：关白附中多糖质量分数达4.62%,平均加样回收率为97.72%,RSD=1.96%.结论该方法实验操作简便,准确可靠,重现性较好.Aconitum coreanum was extracted with hot water,then the crude polysaccharide fraction was obtained by ethanol precipitation. After the freeze-thaw process,the crude polysaccharide was deproteinated by a combination of proteinase and Sevag method. The polysaccharide content in Aconitum coreanum was determined by phenol-sulfuric acid method comparing colors at 490 nm. Results showed that：the polysachardes content in Aconitum coreanum is 4.62% ,the average recovery rate is 97.72% and RSD is 1.96%. This method is simple,the result is stable and reproducible.

关 键 词：关白附 多糖 质量分数测定 硫酸苯酚法 

分 类 号：R284.1[医药卫生—中药学]