肝癌细胞HepG2基因表达谱的构建与分析  

Establishing and Analysis of Gene Expression Profile of Human Hepatocellular Carcinoma Cell Line HepG2

作　　者：金维荣[1] 董辉 张洪义[3] 陈样宜[1] 钱震 孔亚林[3] 沈艳[1] 

机构地区：[1]生物芯片上海国家工程研究中心,上海201203 [2]国家人类基因组南方研究中心,上海201203 [3]空军总医院肝胆外科,北京100036

出　　处：《复旦学报（自然科学版）》2009年第5期656-660,667,共6页Journal of Fudan University：Natural Science

基　　金：上海市自然科学基金资助项目(07ZR14083);国家高技术研究发展计划资助项目(2006AA020704)

摘　　要：为从转录组水平识别HepG2与正常肝组织间的基因表达差异,寻找可能参与肝癌发生发展的重要基因,应用基因表达系列分析技术(SAGE)构建了HepG2的基因表达谱.通过DGED软件进行SAGE标签序列的注释,并与NCBI公共数据库中已有的正常肝脏数据进行比较分析,使用KEGG软件对差异表达基因进行功能分类.共发现HepG2与正常肝组织间差异表达的基因733个,其中表达上调的基因主要与MAPK信号传导通路、细胞周期、细胞粘附等相关,下调基因则主要与烟酸/烟酰胺代谢以及凝血和补体功能相关.荧光实时定量RT-PCR证实了在HepG2中表达升高的IGF2BP2和PEG3基因,在原发性肝癌中的表达亦上调(P<0.05),提示该基因可能是肝癌相关基因.Serial Analysis of Gene Expression（SAGE） was used to obtain the gene expression profile of HepG2 to identify the difference of gene expression between HepG2 and normal liver at transcriptome level as well as find out critical genes involved in hepatocarcinogenesis.733 genes with different expression between HepG2 and normal liver were discovered by annotating SAGE tags using software DGED,comparing against existing normal data of NCBI database,and classifying according to their functions using software KEGG.Up-regulated genes were involved in MAPK signaling pathway,cell cycle,cell adhesion et al,and down-regulated genes mainly participated in nicotinate/nicotinamide metabolism and complement and coagulation cascades.Genes up-regulated in HepG2,such as IGF2BP2 and PEG3 were also detected to exhibit an increased level in HCC confirmed through real-time RT-PCR（P〈0.05）,suggesting potential roles these genes might play in HCC.Our results may provide useful clues for further study on molecular mechanisms of HCC.

关 键 词：基因表达谱 基因表达系列分析 HEPG2 IGF2BP2基因 PEG3基因 

分 类 号：Q786[生物学—分子生物学] Q556.9