硫胺素缺乏早期对成年小鼠认知功能及海马突触长时程增强的影响  

Changes of cognitive function and hippocampal long-term potentiation in adult mice in early stage of thiamine deficiency

作　　者：赵娜[1] 魏文石[1] 李亚健[1] 钟春玖[2] 

机构地区：[1]复旦大学附属华东医院神经内科,上海200040 [2]复旦大学附属中山医院神经内科,上海200032

出　　处：《中华神经医学杂志》2009年第10期1028-1030,1034,共4页Chinese Journal of Neuromedicine

摘　　要：目的研究硫胺素缺乏（TD）早期对成年小鼠认知功能及其海马突触长时程增强（LTP）的影响。方法成年小鼠24只按照随机数字表法均分为实验组和对照组，实验组给予硫胺素剥夺饮食喂养9d制作TD9模型小鼠。对照组则给予含有硫胺素的正常饲料喂养9d。利用Y迷宫检测小鼠的学习记忆能力，利用电生理技术检测小鼠海马CA1及CA3区LTP。结果实验组小鼠出现学习能力的下降，与对照组相比差异有统计学意义（P〈0．05）；记忆功能无显著改变，与对照组相比差异无统计学意义（P〉0．05）。实验组和正常组小鼠海马CA1及CA3区都可以诱导出LTP，两组相比差异无统计学意义（P〉0．05）。结论TD早期损害了小鼠学习能力，其损害机理与海马突触可塑性关系不明显。Objective To investigate the changes of cognitive function and the hippocampal long-term potentiation （LTP） in adult mice in early stage of thiamine deficiency （TD）. Methods Twenty-four adult mice were randomized into normal control group and experimental group, and in the latter group, the mice were given thiamine-depleted diet for 9 days to induce TD. The learning and memory abilities of the mice were tested with Y-maze test, and the LTP in the hippocampal CA1 and CA3 regions was measured electrophysiologically. Results Feeding on thiamine-depleted diet for 9 days significantly increased the total training trials in the learning task as compared with those of the normal control mice （23±0.86 vs 13.50±1.28, P〈0.05）, but the memory ability of the two groups remained comparable （P〉0.05）. By field potential recording in the hippocampal CA1 and CA3 regions, theta burst stimulation induced similarly robust LTP in the control and experimental groups （P〉0.05）. Conclusion The learning ability of adult mice is impaired in early stage of TD, the mechanism of which does not obviously involve hippocampal synaptic plasticity.

关 键 词：硫胺素缺乏 认知功能 海马突触长时程增强 

分 类 号：R686[医药卫生—骨科学]