马尾受压后腰骶部脊髓中P75神经营养因子受体的表达及其与神经元凋亡的关系  

Expression of P75 neurotrophic receptor in lumbosacral spinal cord after cauda equina compression and its relationship with neuron apoptosis

作　　者：李浩鹏[1] 陈杰[1] 贺西京[1] 徐思越[1] 刘宇[1] 

机构地区：[1]西安交通大学医学院第二附属医院骨二科,陕西省西安市710004

出　　处：《中国脊柱脊髓杂志》2009年第10期753-757,共5页Chinese Journal of Spine and Spinal Cord

基　　金：国家自然科学基金资助项目(编号:30672136)

摘　　要：目的:观察大鼠马尾受压后腰骶部脊髓中P75神经营养因子受体(P75 neurotrophic receptor,P75NTR)的表达,探讨P75NTR与前角神经元凋亡的关系。方法:48只雌性成年SD大鼠,随机分为正常组(n=6)、对照组(n=6)和实验组(n=36),正常组不作任何处理,对照组行假手术,实验组在L4平面置入半圆柱体硅胶棒,占椎管截面积约75%～80%,造成马尾急性受压。实验组分别于造模后1d、3d、5d、7d、14d、28d时取L4压迫节段近端脊髓组织(L2水平脊髓),正常组和对照组在第7天时处死取相应部位脊髓组织,切片后进行P75NTR免疫组织化学染色,观察P75NTR的表达;末端转移酶标记技术(TUNEL法)检测脊髓前角运动神经元凋亡情况。用SPSS13.0统计软件进行组间t检验和相关性分析。结果:正常组和对照组脊髓前角有少量P75NTR表达;实验组造模后1d脊髓前角即有P75NTR表达,7d达到峰值,14d、28d表达呈减少趋势,实验组除1d时外其余各时间点与正常组和对照组比较均有显著性差异(P<0.05)。正常组和对照组脊髓组织中可见少量神经元凋亡,实验组造模后1d脊髓前角神经元凋亡数量明显增多,7d达到峰值,14d、28d凋亡呈减少趋势,实验组各个时间点与正常组和对照组比较均有显著性差异(P<0.05)。神经元凋亡率与P75NTR表达存在平行变化关系。结论:马尾受压后脊髓前角运动神经元表达P75NTR,其表达量与神经元凋亡率呈正相关。Objective：To investigate the expression of P75 neurotrophic receptor（P75NTR） at different timepoint in lumbosacral spinal cord after cauda equina compression,and its relationship between it and the apoptosis of anterior horn neurons.Method：48 female adult Sprague-Dawley rats were randomly divided into 3 groups as control group （n=6）,normal group （n=6） and experimental groups （n=36）.The acute cauda equine compression model was established as placing a silicon gel rubber at L4 level of the vertebral canal which represented about 75%-80% compression to the cross section,while the vertebral canals in control group were opened and sutured leaving spinal cord intact.L4 level of spinal cords were harvested at 1d,3d,5d,7d,14d, 28d after operation in experimental group,and at 7d in control group and normal group respectively,and then used immunohistochemistry to observe the expression of P75NTR and TUNEL method was applied to observe cell apoptosis.SPSS 13.0 statistical software was employed to help analysis.Result：There was a less amount of P75NTR expressed in normal group and control group than in test groups,however no significant difference were noted among the unexperimental two groups （P〉0.05）,one day after operation,P75NTR was found expressed in anterior horn neurons of spinal cord in experimental group,and went to peak at 7th days,which decreased in 14d and 28d.There were significant difference with respect to expression of P75NTR between experimental group and control group as well as normal group except datas of ld（P〈0.05）.Small amount of neurons apoptosis were evidenced in normal group and control group,while neurons apoptosis increased remarkably in experimental group,which went to peak at 7th day,and then decreased at 14th day and 28th day.At all six time-points,there were significant differences between experimental group and control group （P〈0.05）, which changed parallely.Conclusion：P75NTR are expressed in anterior horn motor neurons after cauda equina compression,w

关 键 词：马尾综合征 P75神经营养素因子受体 脊髓 神经元 凋亡 大鼠 

分 类 号：R683.2[医药卫生—骨科学] Q513[医药卫生—外科学]