应用luzindole建立双足大鼠脊柱侧凸模型  

Establishment of the scoliosis model in bipedal rats

作　　者：马晓生[1] 吕飞舟[1] 吴俊哲[1] 陈誉[1] 接健[1] 马天文[1] 张权[1] 姜建元[1] 

机构地区：[1]复旦大学附属华山医院骨科,上海市200040

出　　处：《中国脊柱脊髓杂志》2009年第10期774-777,共4页Chinese Journal of Spine and Spinal Cord

摘　　要：目的:探讨应用褪黑素拮抗剂luzindole建立双足大鼠脊柱侧凸模型的可行性。方法:健康刚断乳SD大鼠30只,随机分为A、B、C、D、E共5组,每组6只。A、B、C、D四组通过截去双前肢和尾巴建立双足大鼠模型,A、B、C三组每天18:00～20:00点间腹腔注射褪黑素拮抗剂luzindole,剂量分别为0.2mg/kg、0.5mg/kg、1mg/kg;D组每天相同时间腹腔注射同体积的生理盐水;E组作为空白对照组。观察3个月,每只大鼠每天称重,每间隔1个月摄脊柱全长正位X线片,观察各组大鼠体重变化及侧凸发生情况。结果:A、B、C三组共有7只大鼠发生了脊柱侧凸,发生率为38.9%,其中A组有3只发生了脊柱侧凸,Cobb角为29°～34°,较B、C两组显著(P<0.05);D、E两组均未发生脊柱侧凸。不同时间点5组大鼠体重均有增加,体重增加最多的是注射褪黑素拮抗剂剂量最大的C组。结论:对双足大鼠使用0.2mg/kg褪黑素拮抗剂luzindole能成功建立脊柱侧凸模型;并且可促进大鼠的生长。Objective：To discuss the feasibility and effect of establishing a scoliosis model of bipedal rats by using the antagon of melatonin receptor.luzindole.Method：Thirty healthy and weaning SD rats were divided to five groups randomly with each group of six rats.The animals in groups A, B, C and D were made for bipedal models.The group A,B and C were abdominally given the antagon of melatonin receptor,lnzindole （ip） from 6pro to 8pm every evening.The doses of luzindole were 0.2mg/kg,0.5mg/kg and lmg/kg for three groups respectively.The group D was administrated the same volume of normal saline （ip） contemporarily.The group E made up of quadruped rats were looked as sham.Three months later,all rats were weighted every day and taken X-ray of whole spine every month to note the status of scoliosis.Result：7（38.9%） animals in group A, B and C developed scoliosis.Three rats in group A had developed scoliosis with the curve Cobb＇s angle range of 29°-34°,which was more distinctive than those in group B and C（P〈0.05）,while animals in groups D and E had no scolisis.The animals in group C accepting the highest dose of antagon of melatonin receptor had body weight increasesd most.Conclusion：Use of 0.2mg/kg antagon of melatonin （luzindole） can establish the scoliosis model successfully,and promote the growth of rats as well.

关 键 词：脊柱侧凸 双足大鼠 褪黑素 LUZINDOLE 

分 类 号：R682.3[医药卫生—骨科学] Q576[医药卫生—外科学]