黄瓜叶片细菌性角斑病侵染初期cDNA文库分析  

Analysis of cDNA library of Cucumis sativus L. challenged by Pseudomonas syringae pv. Lachrymans

作　　者：刘关君[1,2] 王丽娟[1] 秦智伟[1] 孟令波[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030 [2]东北林业大学林木遗传育种与生物技术教育部重点实验室,哈尔滨150040

出　　处：《遗传》2009年第10期1042-1048,共7页Hereditas（Beijing)

基　　金：黑龙江省博士后基金资助(编号:LBH-Z07227);中国博士后科学基金(编号:20080440833);国家863计划项目(编号:2007AA10Z177)资助

摘　　要：文章以接种细菌性角斑病原菌48h的抗病品种‘D0462’黄瓜叶片为材料,构建了黄瓜叶片cDNA文库,文库插入片段大小为0.45～2.1kb之间,平均长度为1kb。随机选取2966个克隆进行测序,共拼接出2352个假定独立转录本(TUTs),其中包括282个重叠群(Contigs),2070个单拷贝EST(Singlets)。经BlastX分析共获得已知功能和未知功能基因1848个,无序列相似性新基因504个。文库中含有大量防御/抗病基因,如金属硫蛋白、谷胱甘肽-S-转移酶、泛素、β-1,3-葡聚糖酶、锌指蛋白和半胱氨酸蛋白酶等,这些基因很可能参与了植物与病原菌互作过程。A cDNA library was constructed from the leaves of the disease-resistant cucumber （Cucumis sativus L.） cultivar ＇D0462＇ challenged by Pseudomonas syringae pv. Lachrymans for 48 h. The inserted fragment sizes ranged from 0.45 to 2.1 kb and the average inserted size was 1 kb. Sequencing analysis showed that 2 352 TUTs （Tentative unique transcripts）, 282 contigs, and 2 070 singlets were identified in the 2 966 ESTs derived from the cDNA library. The result of the BlastX analysis indicated that there were 1 848 ESTs with known or unknown function, 504 ESTs with no significant similarity matching with any protein or DNA sequence in the databases. In this library, many defense/disease-resistant related genes, such as metallothionein, glutathione S-transferase, ubiquitin, β-1, 3-glucanase, zinc finger protein, and cysteine protease, which might participate in the plant and the pathogens, are inclued.

关 键 词：黄瓜 叶片 CDNA文库 细菌性角斑病 

分 类 号：S436.421[农业科学—农业昆虫与害虫防治]