植物维管束内寄生难养菌类的提取  

VACUUM EXTRACTION OF FASTIDIOUS PROKARYOTES LIMITED IN PLANT VASCULAR BUNDLE

作　　者：张金岐[1] 张良皖 王祈楷[2] 

机构地区：[1]河北省农林科学院昌黎果树研究所 [2]中国科学院微生物研究所

出　　处：《林业科学》1990年第6期567-570,共4页Scientia Silvae Sinicae

摘　　要：寄生于植物维管束系统引起黄化型病害的病原微生物,目前已知有螺原体(Spiroplasma-Like Organism)。类菌质体(Mycoplasma-Like organism)和难养细菌(Fastidious bacteria)。它们均为原核生物。前二者没有细胞壁属于菌质体类(Mycoplasma);后者在70年代被称为类立克次体或类立克次体细菌(RLO或RLB)。因具有明确的细胞壁结构,而且DNA的G—C比值相当于细菌,现已归入细菌类中。类菌质体至今尚未能在人工培养基中培养,大多数螺原体和少数难养细菌虽已在人工培养基上培养成功。Fastidious prokaryotes limited in vascular bundle of several yellow diseased plants were successfully recovered by means of vacuum extraction and alternative centrifugation. Fastidious bacteria were obtained from apple scar peel disease, pear scar peel disease and persimon proliferation disease. MLO associated with paulownia witches' broom disease was also recovered by this method. Electron micrographs showed the noticeable feature of these prokaryotes.The fastidious bacteria are rod-shaped cell, 0.5—1.0×1.0—3.0μm, with rippled cell walls. The globular wall-less cells with a single unit membrane were observed in the case of paulownia witches' broom. The fastidious bacteria extracted from apple scar peel disease were used as antigen to immunize rabbits. The antiserum obtained reacts obviously with the bacteria recovered from apple and pear plants with scar peel disease. It does not show any visible reaction with the corresponding samples made from healthy plants.

关 键 词：植物 维管束 难养细菌 类菌质体 

分 类 号：S432.42[农业科学—植物病理学]