NYGGF4基因过表达对脂肪细胞胰岛素敏感性及分泌功能的影响  

Effects of NYGGF4 gene over-expression on the insulin sensitivity and secretory function of adipocytes

作　　者：张春梅[1] 邱洁[1] 陈小慧[1] 王玢[1] 张敏[1] 郭锡熔[1] 

机构地区：[1]南京医科大学附属南京市妇幼保健院,江苏南京210004

出　　处：《中国当代儿科杂志》2009年第10期846-849,共4页Chinese Journal of Contemporary Pediatrics

基　　金：国家自然科学基金(编号:30571978);江苏省医学领军人才项目(LJ200624)

摘　　要：目的观察肥胖相关新基因NYGGF4过表达对脂肪细胞的胰岛素敏感性及分泌功能的影响。方法体外培养稳定转染NYGGF4基因(NYGGF4-pcDNA3.1)的3T3-L1前体脂肪细胞,以转染空载体(pcDNA3.1)的3T3-L1细胞为对照,胰岛素加地塞米松加1-甲基-3-异丁基黄嘌呤(MDI)方案诱导细胞分化成熟,液闪仪测定3H标记的葡萄糖摄取率,Westernblot检测葡萄糖转运体4(GLUT4)的表达及转位;采用ELISA双抗体夹心法检测培养上清中TNF-α、IL-6、脂联素、抵抗素4种细胞因子的水平。结果NYGGF4过表达显著降低胰岛素刺激的葡萄糖摄取率(P<0.05)。NYGGF4过表达对总的GLUT4的表达量没有影响,但明显降低胰岛素刺激的GLUT4由细胞浆向细胞膜的转位(P<0.05)。过表达NYGGF4的脂肪细胞其培养上清中TNF-α、IL-6、脂联素及抵抗素的分泌水平与对照组相比差异无显著性(P>0.05)。结论NYGGF4基因过表达通过下调胰岛素刺激的GLUT4的转位而减少脂肪细胞的葡萄糖摄取率,提示脂肪细胞对胰岛素的敏感性降低,而对脂肪细胞的分泌功能无明显影响。Objective To study the effect of a new obesity-related gene NYGGF4 on the insulin sensitivity and secretory function of adipocytes.Methods 3T3-L1 preadipocytes transfected with either an empty expression vector （pcDNA3.1;control group） or an NYGGF4 expression vector （NYGGF4-pcDNA3.1） were cultured in vitro and differentiated into the matured adipocytes with the standard insulin plus dexamethasone plus 3-isobutyl-methylxanthine （MDI） induction cocktail. 2-deoxy-D-[3H] glucose uptake was determined by liquid scintillation counting. Western blot was performed to detect the protein content and translocation of glucose transporter 4 （GLUT4）. The supernatant concentrations of TNF-α,IL-6,adiponectin and resistin were measured using ELISA.Results NYGGF4 over-expression in 3T3-L1 adipocytes reduced insulin-stimulated glucose uptake. NYGGF4 over-expression impaired insulin-stimulated GLUT4 translocation without affecting the total protein content of GLUT4. The concentrations of TNF-α,IL-6,adiponectin and resistin in the culture medium of 3T3-L1 transfected with NYGGF4 were not significantly different from those in the control group.Conclusions NYGGF4 over-expression impairs the insulin sensitivity of 3T3-L1 adipocytes through decreasing GLUT4 translocation and had no effects on the secretory function of adipocytes.

关 键 词：肥胖 NYGGF4 葡萄糖转运体4 3T3-L1脂肪细胞 

分 类 号：R589.2[医药卫生—内分泌]