外源性蛋白酶对肉毒毒素的作用初探  

Preliminary study on the effect of exogenous protease on Botulinum toxin

作　　者：张雪平[1] 何星[1] 李小娟[1] 王云天[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《微生物学免疫学进展》2009年第3期4-7,共4页Progress In Microbiology and Immunology

摘　　要：将B型肉毒毒素在毒素粗提阶段用胰蛋白酶处理,再经浓缩、柱层析和结晶得到纯化的B型肉毒毒素复合物。结果表明:B型肉毒神经毒素经胰蛋白酶处理后单链裂解为双链,在非还原条件下SDS-PAGE显示神经毒素条带,在还原条件下SDS-PAGE只显示轻(L)、重(H)二链条带,而不显示神经毒素条带;纯化后毒素复合物的比活性提高了5.9倍,达到1.60×108LD50/mgPr;HPLC显示活性成分峰面积所占比例增加了9.83%。Botulinum toxin type B was activated with trypsin during extraction, the purified toxin complex was prepared by concentration, chromatography and crystallization. The results showed that after activating with trypsin, the single-chain of botulinum neurotoxin type B molecule is cleaved into the di-chain form; SDS-PAGE show neurotoxin band, but in reduced conditions the band disappear; The specific toxicity of toxin complex is 1.60 × 10^8 LD50/mgPr, increasing 5.9 times; profile of HPLC show active peak area increasing 9.83%.

关 键 词：肉毒梭菌 B型肉毒毒素 胰蛋白酶 

分 类 号：R96[医药卫生—药理学]