用多种探针药物评价穿心莲对鸡药物代谢酶活性的影响  

Evaluation of influence of Andrographis paniculata on activities of CYP1A2,CYP3A4,CYP2E1 and CYP2C19 genotypes in chickens by multiple cytochrome P450 probe substrates

作　　者：刘开永[1,2] 黄显会[1] 高海[1,3] 贺利民[1] 曾振灵[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]河南科技大学食品与生物工程学院,河南洛阳471003 [3]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国兽医科学》2009年第9期825-829,共5页Chinese Veterinary Science

基　　金：广东省自然科学基金项目(04105984)

摘　　要：采用咖啡因、氨苯砜、氯唑沙宗、奥美拉唑药物探针法,测定这些探针药物在鸡体内的消除半衰期,以探讨穿心莲超微粉对鸡的药物代谢酶的影响,结果显示,对照组和试验组的咖啡因t1/2β分别为632min、415 min,表明两组的t1/2β之间有显著差异(P<0.05)。对照组与试验组的氨苯砜t1/2β分别为55.4 min、48.4 min,二者之间差异不显著(P>0.05)。对照组和试验组的奥美拉唑t1/2β分别为16.5 min、7.6 min,表明两组的消除半衰期差异极显著(P<0.01);氯唑沙宗t1/2β分别为26.3 min、20.5 min,两组差异显著(P<0.05)。结果表明,穿心莲超微粉对CYP3A4、CYP1A2、CYP2E1和CYP2C19的活性都有诱导作用,但与对照组相比,对CYP3A4的诱导作用差异不显著,而对CYP2C19的诱导作用差异极显著。To study the effects of Andrographis paniculata ultra-fine powder on drug metabolic enzymes in chickens, the four drug probes,caffeine, aminophenylsulfone,omeprazole and chlorzoxazone in vivo ,were used in the tests,and the elimination half time of the approaches was determined. The t1/2β in the control group and test group of caffeine was 632 rain and 415 rain respectively, and there was significantly difference between them（P〈 0.05）. The counterparts of aminophenylsulfone, omeprazole and chlorzoxazone were 55.4 and 48.4 min（P〉0.05）,16.5 and 7.6 min（P〈0.01）,26.3 and 20.5 rain（P〈0.05）, respectively. The conclusions were that Andrographis paniculata ultra-fine powder could induce the metabolic enzyme activities of CYP1A2, CYP2E1 and CYP2C19 significantly, CYP3A4 not significantly, CYP2C19 in strongly-marked differences.

关 键 词：穿心莲超微粉 代谢酶 多药物探针法 鸡 

分 类 号：S859.79[农业科学—临床兽医学]