泡桐组培苗VA菌根的研究  

A STUDY ON VA MYCORRHIZAE FORMED ON THE TISSUE-CULTURED PLANTLETS OF PAULOWNIA

作　　者：郭秀珍[1] 赵志鹏[1] 毕国昌[2] 

机构地区：[1]中国林业科学研究院林业研究所 [2]中国科学院研究生院

出　　处：《林业科学研究》1990年第1期9-14,共6页Forest Research

基　　金："七五"国家重点攻关科技项目

摘　　要：本文报道了用地表球囊霉对鄂川泡桐和白花泡桐的组培苗进行人工接种,培育菌根化苗木的试验。观察了菌根的发育和分布,并用X射线能谱仪对这种VA菌根的不同超微结构和不同部位进行了微区分析。经测定,地表球囊霉对泡桐组培苗有显著的生长促进效益,形成的VA菌根对P、S、Mg和Ca等元素的吸收都比对照苗要高得多。在菌根内部这些营养元素的分布也不均匀。P、S的含量在丛枝中最多,在胞内泡囊中较少,而在无菌丝感染的根毛细胞中最少。Tissue-cultured plantlets of 2 Paulownia species (Paulownia fortunei (Seem.) Hemsl. and P. albiphloea Z. H. Zhu. sp. nov.) were inoculated with Glomus epigaeum Daniels & Trappe in containers containing mixed substrate of peat-vermiculite-sand-soil (2:1:1:1 v/v). After 30 days of inoculation, growth of plantlets between the inoculated and uninoculated treatments showed a significant difference in shoot height, collar diameter and foliage area, but there was no difference between the species. The observation of vertical distribution of VA mycorrhizae on the root system of Paulownia seedlings showed that most of VA mycorrhizae were concentrated in the top 10 cm soil layer. The morphology and fine structures of these mycorrhizae were also studied by light and scanning electron microscopes. The distribution of several nutrient elements in these mycorrhizae was detected with X-ray microanalysis, spectra of which showed that arbuscules, vesicles and hyphae contained more P, S, Mg and Ca than the uninoculated plantlets did.

关 键 词：泡桐 育种 组培苗 VA菌根 

分 类 号：S792.43[农业科学—林木遗传育种]