对构建的家蚕抗核型多角体病毒病近等基因系的评估  

Evaluation of a Constructed Near-isogenic Silkworm Line with Nucleopolyhedrovirus Resistance

作　　者：刘晓勇[1] 姚勤[1] 陈克平[1] 

机构地区：[1]江苏大学生命科学研究院,江苏镇江212013

出　　处：《江苏农业学报》2009年第5期986-990,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家重点基础研究发展计划"973"项目(2005CB121000);国家"863"计划(2008AA10Z145)

摘　　要：为了从DNA、RNA和蛋白质水平上研究家蚕抗核型多角体病毒病的抗性基因紧密连锁的分子标记及相关的抗性基因和蛋白,选用抗性和感性的亲本,通过杂交和回交技术构建了近等基因系。为了从分子水平上评估这个模型的准确性,尤其是对构建的抗性近等基因系与感性的轮回亲本之间遗传背景的高度相似性的评估。本研究中我们对随机多态性DNA和双向电泳的一些结果进行了分析,比较了以前构建的近等基因系和轮回亲本之间在DNA和蛋白质水平的差异,证明其相似性虽比理论值低,但这个模型对研究抗性仍然是有价值的,对研究家蚕其他的差异基因是有借鉴作用的。To find out the molecular markers closely linked with nucleopolyhedrovirus resistant gene, and resistance related genes and proteins on DNA, RNA and protein levels, a near-isogenic silkworm line was constructed with nueleopolyhedrovirus resistant and susceptible parents through hybridization and backcross. The accuracy of the model, especially the resemblance of genetic background between the NIL and susceptible recurrent partent was evaluated through analysis of the results of RAPD and two-dimensional polyacrylamide gel electrophoresis. The difference between NIL and its recurrent parent indicated that the model was helpful for resistance reseaches despite its lower resemblance.

关 键 词：家蚕 家蚕核型多角体 近等基因系 随机多态性DNA 

分 类 号：Q969.435.103[生物学—昆虫学]