苯胺类席夫碱对昆虫酚氧化酶的抑制作用  

Inhibitory Effects of Schiff Analogues of Aniline on Phenoloxidase from Pieris rapae(L.)and Plutella xylostella(L.)

作　　者：肖婷[1,2] 罗万春[2] 潘以楼[1] 

机构地区：[1]江苏丘陵地区镇江农业科学研究所,江苏句容212400 [2]山东农业大学植物保护学院农药毒理与应用技术省级重点实验室,山东泰安271018

出　　处：《江苏农业学报》2009年第5期1010-1014,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30571237)

摘　　要：为筛选新型害虫控制剂,以9种苯胺类席夫碱化合物为效应物,研究了其对菜青虫酚氧化酶活力的影响,并通过浸渍法进行活体测定。结果表明:所试9种化合物对菜青虫酚氧化酶均有一定的抑制活性,以2-羟基-4-甲氧基苯甲醛缩间氯苯胺、2-羟基-3-甲氧基苯甲醛缩苯胺、2-羟基-3-甲氧基苯甲醛缩3,4-二氯苯胺3种化合物抑制效果较好,其抑制中浓度(IC50)分别为0.051 mmol/L、0.013 mmol/L和0.059 mmol/L。动力学研究结果表明,这3种化合物对酚氧化酶的抑制作用均表现为可逆竞争性抑制。室内活体生测结果表明,这3种化合物对小菜蛾直接"杀死"活性均不高。To screen the insecticide with novel modes of action, the inhibitory effects of 9 aniline schiff analogues as inhibitors on PO from Pieris rapae was studied by using microtitration assay, then a bio-assay was conducted by the immersion method in lab. The results showed that the tested compounds 2-hydroxy-4-methoxybenzylidene-3-chloroaniline, 2-hydroxy-3-methoxybenzylidene-anitine,2-hydroxy-3-methoxybenzylidene-3,4-diehloroaniline could inhibit PO activity obviously and the inhibitory concentration（ IC50 ） was estimated to be 0. 051 mmol/L,0. 013 mmoL/L and 0. 059 mmol/L, respectively. The inhibitors were all found to be reversible competitive inhibitors. Bio-assay of the 3 inhibitors for controlling Plutella xylostella was carried out and the results showed that the ＂killer＂ effects on Plutella xylostella was not obvious.

关 键 词：苯胺类席夫碱 酚氧化酶 抑制剂 抑制机理 室内生测 

分 类 号：S436.341.2[农业科学—农业昆虫与害虫防治]