两株猪圆环病毒2型病毒的分离鉴定及其序列分析  

Isolation and Identification of Two Porcine Circovirus Type 2 Strains and Sequence Analysis of Their Genomes

作　　者：郭容利[1] 何孔旺[1] 钟书霖[1] 温立斌[1] 潘群兴[1] 

机构地区：[1]江苏省农业科学院兽医研究所,农业动物疫病诊断与免疫重点开放实验室,国家兽用生物制品工程技术研究中心,江苏南京210014

出　　处：《江苏农业学报》2009年第5期1063-1067,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家“973”计划前期专项(2007CB116308);江苏省农业科技自主创新资金项目[CX(07)608];江苏省农业科学院基金项目(6110736)

摘　　要：利用PK15细胞从猪断奶后多系统衰竭综合征（PMWS）临床发病与死亡猪中分离到2株病毒；分别命名为Haian（登录号为FJ712216）和Xuancheng（登录号为FJ712215）。利用间接免疫荧光（IFA）和聚合酶链式反应（PCR）对其进行鉴定，并对其全基因组序列进行了测定和分析。结果表明，分离株感染细胞后病毒抗原主要分布在细胞核中，2分离株的基因组全长为1767nt，与世界上其他地区的PCV2分离株密切相关，核苷酸序列同源性达93．7％-97．7％，属于毒力较强的Genotype1。Two porcine cireovirus type 2 （PCV2） strains, named Haian （FJ712216） and Xuancheng（ FJ712215 ）, were isolated from lymphoid node samples of clinically postweaning mnhisystemic wasting syndrome （PMWS） infected and dead pigs by PK15 cell culture. The results showed the two strains of PCV2 distributed mainly in the infected cell nucleolus by indirect immunofluorescence assay （IFA） ; The complete genomes of the two isolated strains were sequenced and ana- lyzed. The results showed that their genomes were both 1 767 nt in length. The sequences are closely related to those of other PCV2 isolates in the GenBank ,and the homology was 93.7% -97.7%. It was presumed that the two isolated strains were high pathogenic PCV2 genotype 1.

关 键 词：猪圆环病毒2型 分离 鉴定 序列分析 

分 类 号：Q78[生物学—分子生物学]