硫酸化淫羊藿多糖对鸡外周血淋巴细胞IL-2和IFN-γ的mRNA表达的影响  

Effects of Sulfated Epimedium Polysaccharides on mRNA Expression of Interleukin-2 and Interferon-γ in Chicken Peripheral Lymphocyte

作　　者：卢宇[1,2] 王凯民[3] 郭振环[1] 张帆[1] 王德云[1] 胡元亮[1] 

机构地区：[1]南京农业大学中兽医学研究室,江苏南京210095 [2]江苏省农业科学院,国家兽用生物制品工程技术研究中心,江苏南京210014 [3]江苏省出入境检验检疫局,江苏南京210001

出　　处：《江苏农业学报》2009年第5期1073-1077,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30571360)

摘　　要：促进淋巴细胞分泌抗病毒干扰素和白介素等细胞因子是生物活性多糖抗病毒和增强免疫的机制之一。为探明sEPS5和sEPS2抗病毒和增强免疫作用的机理,用Primer Premier软件设计了1对白介素-2(Interleukin-2,IL-2)引物和1对干扰素-γ(Interferon-γ,IFN-γ)引物,用荧光定量PCR方法测定sEPS2和sEPS5对鸡外周血T淋巴细胞IL-2、IFN-γ的mRNA表达的影响,以未修饰的EPS为对照。结果表明,在62.50μg/ml和31.25μg/ml时,sEPS5和sEPS2对2种细胞因子的诱生作用显著强于未修饰的EPS,其中sEPS5的作用最强,sEPS2次之,并与剂量呈正相关。这可能是硫酸化多糖抗病毒和增强免疫的分子机制之一。To promote the secretion of interferon, interleukin and many other cytokines was one mechanism of the anti-virus and immune-enhancement actions of bioactive polysaccharides. In order to study the molecular mechanism of sEPS2 and sEPS5 in anti-virus and immune-enhancement actions, according to Primer Premier Software, two pairs of RT- PCR primers of IL-2 and interferon-γ （IFN-γ） were designed. The effects of sEPS5 and sEPSz on the expression level of IL-2 and IFN-γ mRNA,isolated from ConA-stimulated peripheral blood T lymphocyte of chicken, were determined by fluorescence quantitative PCR assay, taking EPS as control. The results showed that sEPS2 and sEPS5 could improve the expression level of IL-2 and IFN-γ mRNA, and were significantly better than EPS at 62. 5 ug/ml and 31.25 ug/ml. The action of sEPS5 was the strongest and in a dose-dependant manner. It might be one of the immunoenhaneement mechanisms that polysaccharides could promote the secretion of IL-2 and IFN-γ.

关 键 词：淫羊藿多糖 硫酸化修饰 鸡 淋巴细胞 细胞因子 荧光定量PCR 

分 类 号：S858.31[农业科学—临床兽医学]