极端嗜热微生物分子伴侣蛋白研究与嗜热功能蛋白质的规模化制备技术研究  

Molecular Chaperones in Hyperthermophiles and Techniques Involved in the Large-scale Production Process of Recombinant Proteins

作　　者：张毅[1] 

机构地区：[1]中国科学院上海生命科学研究院生物工程中心,上海200031

出　　处：《中国基础科学》2009年第5期86-89,共4页China Basic Science

基　　金：国家重点基础研究发展计划(2004CB719606)

摘　　要：极端微生物是丰富的资源宝库,有着巨大的生物技术开发前景。本文从特殊功能蛋白质的发现与表征、结构与功能及潜在性应用,以及特殊功能蛋白质的规模化制备技术两个方面,对极端微生物的资源开发与利用进行了探讨。重点介绍了极端嗜热古菌Pyrococcus furiosus分子伴侣蛋白系统的一些研究,并以P.furiosus胞外α-淀粉酶PFA为例,对工业生物技术领域重组蛋白质的规模化制备技术进行了讨论。Extremophiles is a rich resource with great biotechnological potentials. In this article, we have discussed the developments and applications of the extremophiles resources from two aspects： the discoveries and characterizations of the functional proteins, the structure-function relationships of the target proteins and their potential applications; techniques involved in the large scale production process of the recombinant proteins with special interests. Studies on the molecular chaperone proteins in the hyperthermophilic archaeon P. furiosus are introduced in detail. The extra-cellular alpha-amylase from P. furiosus is used as an example in the discussion of the studies of the technologies involved in the scaleproduction processes of the recombinant proteins with industrial interests.

关 键 词：极端微生物 分子伴侣蛋白 嗜热蛋白质 重组蛋白质的规模化制备技术 

分 类 号：Q81[生物学—生物工程] Q93