蝮蛇蛇毒PCA对实验性心肌梗死大鼠血凝状态的影响  

Protein C activator from venom of agkistrodon halys on coagulation of experimental myocardial infarction rats

作　　者：孔岩[1] 李曙[2] 张根葆[2] 吴娟[1] 

机构地区：[1]皖南医学院蛇毒研究室,安徽芜湖241002 [2]皖南医学院病理生理学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2009年第5期316-318,F0003,共4页Journal of Wannan Medical College

基　　金：安徽省高校青年教师自然科学基金项目(2007jp1172)

摘　　要：目的:探讨蝮蛇蛇毒蛋白C激活剂(PCA)对心肌梗死发生后血液凝固性的影响及其机制。方法:SD大鼠24只,随机分为对照组、心肌梗死模型组和PCA实验组。动物麻醉后,从心尖部向左心室注射月桂酸钠(1.0mg/kg)复制心肌梗死(AMI)模型。实验组大鼠于造模前由舌下静脉注射PCA(1.0mg/kg)预处理,由Medlab生物信号采集系统监测心电变化。于造模后30min从颈总动脉取血样2ml,以TEG5000型血栓弹力仪测量各样本血液的凝血时间、血凝块形成时间、Alpha角度、凝血最大幅度和凝血指数等参数。结果:模型组大鼠血样的R值较对照组明显缩短(P<0.01),CI值>+3.0,而Alpha角度变化不大;LY30%值明显增大,但小于7.5%。PCA实验组大鼠血样的R值较模型组明显延长,MA值减小(P<0.05),LY30%、A值、CI值明显减少(P<0.01)。结论:心肌梗死大鼠血液表现显著高凝状态,蛇毒PCA组分的预处理可以减轻这一过程。Objective ： To investigate the effects and mechanisms of protein C activator （PCA） from the venom of agkistrodon halys on blood coagulation after the myocardial infarction. Methods：Twenty-four SD rats were randomly divided into control, myocardial infarction （AMI） or protein C activator （PCA） group. After anesthesia,the rats were given injection of sodium laurate （1.0 mg / kg） by the apex into left ventricle for replicating the model of acute myocardial infarction （ AMI ）. PCA group were administered PCA（ 1.0 mg/kg） by sublingual intravenous injection before model replication, with ECG monitoring by Medlab bio-sigual acquisition system. Thirty minutes after the animal models were prepared, blood samples of 2 ml were taken the from carotid arteries for measuring the blood with regard to time of coagulation reaction and formation, rate of blood coagulation speed（ alpha）, coagulation strength and index by thromboelastography TEG5000 （ Haemoscope, US）. Results： The R value in AMI group reduced significantly as compared with the controls （ P 〈 0.01） and CI values were greater than ＋ 3. 0, but the Alpha angle changed little with the MA value. LY30% was significantly increased,but was less than 7.5%. PCA group had much longer R value of blood than the model group,with reduced MA value （ P 〈 0.05 ）, LY30%, A value and CI （P 〈0.01 ）. Conclusion ： Rats with AMI are characterized by hypercoagulability,but the findings suggest that venom PCA could reduce the incidence of the coagulation.

关 键 词：蛇毒 蛋白C激活物 血栓弹力图 大鼠 心肌梗死 

分 类 号：R996.3[医药卫生—毒理学]