不同品种猪生长激素基因的PCR-RFLP分析  

PCR-RFLP analysis of porcine growth hormone gene in different porcine breed

作　　者：王荣梅[1] 苏荣胜[2] 张细权[3] 

机构地区：[1]韶关学院农业科学系,广东韶关512005 [2]华南农业大学兽医学院,广东广州510642 [3]华南农业大学动物科学学院,广东广州510642

出　　处：《韶关学院学报》2009年第9期85-88,共4页Journal of Shaoguan University

基　　金：国家重大基础研究规划项目(G2000016102)

摘　　要：采用PCR-RFLPs技术,对临高猪、玉山黑猪、蓝塘猪及国外猪种的生长激素基因-119～+486区域共605 bp片段的扩增产物用Apa I限制性内切酶检测酶切位点的多态性.结果显示:Apa I酶切时,四个品种的猪都出现了3种基因型(AA、BB和AB),等位基因B的频率以蓝塘猪最高,等位基因A的频率以玉山黑猪最高,四个猪种间基因型频率、等位基因频率的差异不显著(P>0.05).Apa I polymorphisms in porcine growth hormone （pGH） gene＇s a 605bp segment encompassing base - 119 through base ＋ 486 were analyzed by PCR - RFLP in Lin-gao Pig ,Yushan Black Pig,Lan-tang Pig and Foreign Pigs.The results showed that there were three genetypes（AA , BB and AB） in every porcine breed when the PCR products were cut by Apa I, BB genotype frequency of Lan-tang Pig was highest in four breeds and AA genotype frequency of Yu-shan Pig was highest in four breeds , and the difference of genotype frequencies and allele frequencies in four breeds was not significant （P〉0.05）.

关 键 词：猪生长激素 基因 ApaI PCR-RFLP 

分 类 号：S828.89[农业科学—畜牧学] Q575.11[农业科学—畜牧兽医]