HPLC法测定麒麟丸中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量  

Determination of 2,3,5,4'-Tetrahydroxy Chrysophenine-2-O-β-D-Glucoside in Qilin Pill by HPLC

作　　者：江秀娟[1] 陈新[1] 梁华伦[2] 

机构地区：[1]广州市药品检验所二分所,广东广州510250 [2]广东省中医院,广东广州510515

出　　处：《亚太传统医药》2009年第10期28-30,共3页Asia-Pacific Traditional Medicine

摘　　要：目的:建立麒麟丸中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定方法。方法:采用HPLC法测定,色谱柱为Dikma Diamonsil,(C18,250mm×4.6mm,5μm),流动相为乙腈—水(14:86),流速1.0mL/min,检测波长320nm。结果:2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.0491～1.473μg之间呈线性关系,r=0.9996,平均回收率为95.8%,RSD为1.3%。结论:该方法简便准确,重现性好,可用于麒麟丸的质量控制。关键词:麒麟丸;高效液相色谱法;2,3,5。Objective：To establish a HPLC method for the determination of 2,3,5,4 -tetrahydroxy chrysophen-ine-2-O-β-D-glucoside in Qilin pill.Methods：The analytical column was a Dikma Diamonsil （C18250×4.6mm,5μm）,and the mobile phase was acetonitrile-water （14：86）with a flow rate of 1.0ml/min,The detector wavelength was 320 nm.Results：2,3,5,4 -tetrahydroxy chrysophenine-2-O-β-D-glucoside curve was linear over the range of 0.0491～1.473μg （r=0.9996）.The average recovery rate was 95.8%,RSD was 1.3%（n=6）.Conclusion：The method is simple,accurate,reproducible,and can be used for the quality control of Qilin pill.

关 键 词：麒麟丸 高效液相色谱法 2 3 5 4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 

分 类 号：R284.2[医药卫生—中药学]