细叶桉遗传多样性的ISSR分析  

Genetic Diversity of Eucalyptus tereticornis by ISSR

作　　者：张党权[1] 田华[1] 谢耀坚[2] 谭晓风[1] 黄青云[1] 谷振军[1] 曹家光[2] 曾艳玲[1] 

机构地区：[1]中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004 [2]国家林业局桉树研究开发中心,广东湛江524022

出　　处：《中南林业科技大学学报》2009年第5期91-94,共4页Journal of Central South University of Forestry & Technology

基　　金：长沙市科技计划项目重点课题(K069083-22);湖南省教育厅科学研究项目(08C931)

摘　　要：利用ISSR分子标记技术对细叶桉的遗传多样进行了分析.从100条随机引物中筛选出10条特异的用于细叶桉ISSR-PCR扩增引物,扩增出101条清晰可重复的条带,其中89条是多态条带,多态带百分率为86.1%.采用POPGENE软件,计算了种间的Nei氏遗传距离,并用UPGMA法构建了聚类分析树形图,分析了它们的遗传多样性和亲缘关系.The genetic diversity of Eucalyptus tereticornis was analyzed by ISSR technology.According to the improved ISSR-PCR system,10 primers were selected out of 100 random ones to yield specific bands with moderate band numbers,clear background and good reproducibility.Totally,101 specific and reproducible DNA bands were obtained,of which 89 are polymorphic bands（86.1% of polymorphism）.Bio-software POPGENE 1.31 and NTSYS-pc were used to calculate the Nei genetic distance and produce the UPGMA dendrogram of genetic diversity and similarity.

关 键 词：生物技术 细叶桉 ISSR 遗传多样性 聚类分析 

分 类 号：Q754[生物学—分子生物学]