PAB和顺铂联用抑制HO-8910裸小鼠移植瘤生长作用研究  

Investigation on inhibiting effect of pseudolaric acid B and cisplatin on growth of HO-8910 xenografted tumors in nude mice

作　　者：李克深[1] 霍贵成[1] 胡云[2] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]解放军第211医院妇产科

出　　处：《中国妇幼保健》2009年第30期4279-4281,共3页Maternal and Child Health Care of China

基　　金：黑龙江省博士后基金资助项目〔LBH-Z06159〕;黑龙江省自然科学基金资助项目〔AD200647〕

摘　　要：目的:探讨土荆皮酸(PAB)和顺铂联用对人卵巢癌细胞株HO-8910裸鼠皮下移植瘤的生长及其血管生成的影响。方法:将20只荷人HO-8910卵巢癌的裸小鼠随机分成4组:①PAB组(60 mg/kg,腹腔注射治疗5天);②顺铂组(5 mg/kg,腹腔注射治疗5天);③联合用药组(PAB 60 mg/kg+顺铂5 mg/kg,腹腔注射治疗5天);④阴性对照组(给予同体积的含有6%聚乙二醇、3%乙醇、1%Tween 80的水溶液);计算瘤质量、瘤体积;同时应用CD34免疫组化染色,观测瘤组织内的微血管密度。结果:统计学分析表明:在抑瘤率和微血管密度方面,PAB组、顺铂组和阴性对照组比较差异具有统计学意义(P<0.01),联合用药组和其他3组比较差异均有统计学意义(P<0.01)。结论:PAB、顺铂对人卵巢癌移植瘤的生长具有抑制作用;PAB和顺铂联用能起增效作用。Objective：To explore the effect of pseudolaric acid B（PAB）and cisplatin on growth and angiogenesis of xenografted tumor of human ovarian cell line HO-8910 in nude mice.Methods：20 nude mice bearing human ovarian carcinoma HO-8910 xenografted tumor were divided into 4 groups randomly： ①PAB group（injected by 60 mg/kg PAB for 5 days）;②cisplatin group（injected by 5 mg/kg cisplatin for 5 days）;③combination group（injected by 60 mg/kg PAB and 5 mg/kg cisplatin for 5 days）;④control group（same volume of solution containing 6% polyethylene glycol,3% ethanol and 1% Tween 80）;the tumor volume and weight were measured;the microvessel densities（MVDs） in four groups were detected by CD34 immunohistochemical staining.Results：There was difference in inhibition rate of tumors and MVD between PAB group,cisplatin group and control group（P〈0.01） as well as between combination group and the other 3 groups（P〈0.01）.Conclusion：PAB and cisplatin can inhibit tumor growth of nude mice bearing human ovarian carcinoma cell line HO-8910 and their combination can produce a synergistic action.

关 键 词：土荆皮酸 顺铂 卵巢癌细胞 移植瘤 血管生成 

分 类 号：R979.1[医药卫生—药品]