基质金属蛋白酶-8及组织抑制物-1与胎膜早破的关系  

Relationship between matrix metalloproteinase-8/tissue inhibitor of matrix metalloproteinase-1 and premature rupture of membranes

作　　者：贺喜风[1] 杨海澜[1] 韩方[1] 武桂花[1] 郝敏[1] 

机构地区：[1]山西医科大学第一医院产科,山西太原030001

出　　处：《中国妇幼保健》2009年第30期4281-4284,共4页Maternal and Child Health Care of China

摘　　要：目的:探讨孕妇胎膜基质金属蛋白酶-8(MMP-8)及金属蛋白酶组织抑制物-1(TIMP-1)的水平变化以及与胎膜早破的关系。方法:30例胎膜早破孕妇作为胎膜早破组,其中妊娠37～41周18例,28～36+6周12例。30例非胎膜早破孕妇作为对照组,平均妊娠34～41+2周。应用免疫组织化学SP法和图像分析法检测两组孕妇胎膜MMP-8和TIMP-1水平,比较胎膜早破组与对照组在这些指标上的差异。结果:①胎膜早破组和对照组两组孕妇胎膜MMP-8的阳性表达分别为100%和70%,胎膜早破组水平明显高于对照组,差异有统计学意义(P<0.01);②胎膜早破组和对照组两组孕妇胎膜TIMP-1的表达,胎膜早破组低于对照组,但差异无统计学意义(P>0.05)。结论:MMP-8水平的异常升高及TIMP-1水平的下降,可能是胎膜早破重要的发病机制。Objective：To explore the changes of matrix metalloproteinase 8（MMP-8） and tissue inhibitor of matrix metalloproteinase-1（TIMP-1） levels in fetal membranes of pregnant women and their relationship with premature rupture of membranes（PROM）.Methods：30 pregnant women with PROM were enrolled in the study as PROM group（18 cases of 37～41 gestational weeks and 12 cases of 28～36＋6 gestational weeks）.30 pregnant women without PROM（34～41＋2gestational weeks on average） were recruited as control group.MMP-8 and TIMP-1 levels in the two groups were detected by immunohistochemistry S-P method and computer image analysis system.Results：①The positive rates of MMP-8 in fetal membranes in PROM group and control group was 100% and 70%,respectively,there was significant difference（P〈0.01）.The expression rate of TIMP-1 in fetal membranes of PROM group was lower than that in control group without difference（P〈0.05）.Conclusion：It is suggested that PROM is associated with increased MMP-8 and decreased TIMP-1 levels.MMP-8 and TIMP-1 are valuable clinical biological markers for identifying PROM combined with chorioamnionitis.

关 键 词：基质金属蛋白酶-8 基质金属蛋白酶组织抑制物-1 胎膜早破 

分 类 号：R714[医药卫生—妇产科学]