氧化铝羟基磷灰石复合陶瓷修复兔桡骨节段性缺损的影像学观察  

Iconography Observation in Segmental Bone Resection Defect Repairing Using Al_2O_3/HA Ceramic

作　　者：赵燚[1] 徐武清[2] 马英锋[2] 赵喆[3] 王倩秋 墙华[5] 王荒野[6] 赵自强[2] 

机构地区：[1]宁夏医科大学公共卫生学院,银川750004 [2]宁夏医科大学中医学院,银川750004 [3]瑞典斯德哥尔摩大学 [4]宁夏友谊慈善医院X光室,银川750004 [5]宁夏医科大学第二附属医院,银川750001 [6]宁夏医科大学临床医学院动物外科实验室,银川750004

出　　处：《宁夏医科大学学报》2009年第5期570-573,F0002,共5页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金(E006-2004);宁夏国际合作项目05GJ-11400)2个基金资助

摘　　要：目的评价放电等离子烧结技术制备的氧化铝羟基磷灰石复合陶瓷修复兔桡骨节段性缺损的效果。方法选新西兰白兔15只,制作10mm长的桡骨节段性缺损模型,分为实验组:15个肢体,骨缺损区植入氧化铝羟基磷灰石复合陶瓷人工骨材料;空白对照组:6个肢体,骨缺损区旷置;自体骨对照组:6个肢体,骨缺损区植入自体骨。术后1d,8、16、24周分别进行X线摄片观察,运用ImageTool图像处理软件,进行点像素测定,24周进行扫描电镜观察。结果实验组:氧化铝羟基磷灰石复合陶瓷人工骨植入兔桡骨缺损区8周即有新生骨形成,24周材料与骨组织连接在一起,缺损区得以修复。空白组:8周时缺损区有少量骨痂,24周时修复缺损区约1/2;自体骨植入组:8周时骨断端完全被骨痂包绕,24周时缺损区骨完全修复。像素测量结果显示三组间骨修复速度差异无统计学意义(P>0.05);重复测量资料的方差分析P=0.007,说明随着时间的延长骨组织密度增加。实验组材料两端修复界面新生骨与皮质骨的X射线摄片像素值进行比较:术后1d,8、16周P<0.05,24周P>0.05。说明术后24周前材料两端界面在不断修复,24周时完全修复。扫描电镜摄片示:实验组兔植入材料被新骨组织环形包绕与骨组织镶嵌,皮质骨与界面骨之间形成骨髓腔;在材料表面凹陷中骨细胞环状排列形成骨单位。结论证实采用放电等离子烧结技术制备的氧化铝羟基磷灰石复合陶瓷材料能够修复新西兰兔桡骨节段性缺损,具有良好的生物活性和骨传导性。Objective To evaluate the effect of using Al2O3/HA composite to repair the segmental bone resection defect in rabbit. Methods A total of 15 New Zealand rabbits were prepared for rabbit radius defect models and randomly divided into 3 groups： rabbits in the experimental group （n = 6） were implanted with the prepared Al2O3/ HA material; animals in the blank control group （n = 6） were prepared segmental bone resection surgery without implant material; rabbits in the self-control group （n = 6） were implanted with the autogenous bone block from segmental bone resection surgery after washing with normal saline. After 1 day, 8,16,24 weeks, respectively, for X-ray observation, ImageTool image processing software was used to measure pixel-point, then, Scanning electron microscopy was measured after 24 weeks. Results Al2O3/HA ceramic composite artificial bone, which were prepared by spark plasma sintering, implanted in rabbit radial bone defects. There was new bone formation after 8 weeks. After 24 weeks, the material connected with the bone tissue, and the defects was healed. Blank Group： A small amount of callus were formed in bone defect area after 8 weeks, and it repaired the 1/2 defect area after 24 weeks. Self-control Group： The bone ended completely enveloping callus at 8 weeks and the defect area was completely repaired at 24 weeks. Pixel measurement results showed that there was no difference in the speed of bone repairs （ P 〉 0.05） among the three groups; other statistical result showed that the density of bone tissue was increased with the extension of time （ P = 0.007）. We found that there were no difference in radiography x-ray pixel value between new bone and cortical bone （P 〉 0.05） on the end of materials in experimental groups at 1 day 8 weeks and 16 weeks, the result showed that the end of materials was constantly repaired after surgery and the defects area was healed at 24 weeks. Scanning electron microscope X-ray film showed that materials was enveloped by the new bone

关 键 词：羟基磷灰石类 影像学 骨缺损 兔 

分 类 号：R687[医药卫生—骨科学]