东乡族人G894T多态性与坎地沙坦降压疗效的研究  

作　　者：闵海 马丽雅[2] 张辉锋[3] 黄德珍 

机构地区：[1]甘肃省临夏州人民医院,731100 [2]甘肃省人民医院,730000 [3]兰州大学第一临床医学院,730000 [4]东乡自治县人民医院,731400

出　　处：《当代医学》2009年第31期20-22,共3页Contemporary Medicine

基　　金：甘肃省科技厅资助项目(项目编号:0708NKCN098)

摘　　要：目的探讨原发性高血压患者内皮型一氧化氮合酶(eNOS)基因G894T多态性与坎地沙坦降压疗效的相关性。方法(1)入组甘肃省东乡族203例原发性高血压患者,安慰剂治疗2周后,服用坎地沙坦单药4周,观察并记录疗效。(2)试剂盒抽提DNA,应用聚合酶链反应-限制性内切酶片段长度多态分析法对高血压患者的eNOS第7外显子G894T进行基因多态性分型,并测定体重指数、空腹血糖、血浆甘油三酯和胆固醇水平。结果(1)eNOS基因G894T多态性符合Hardy-Weinberg平衡定律;G、T等位基因的频率分别为0.8571和0.1429。(2)治疗前T等位基因携带者的收缩压(165±14.2)mmHg、舒张压(97±10.5)mmHg与GG基因型携带者对应收缩压(164±13.9)mmHg、舒张压(96±11.2)mmHg相比较差异无统计学意义(P>0.05)。治疗4周后GG基因携带者的收缩压下降(13.6±6.6)mmHg、舒张压下降(11.4±4.3)mmHg,分别大于T等位基因型收缩压下降(9.2±5.7)mmHg、舒张压下降(8.5±4.4)mmHg,且差异均有统计学意义(P<0.05)。结论东乡族人群高血压患者eNOS基因G894T多态性与坎地沙坦降压疗效有相关性。Objective To investigate the association of the G894T polymorphism of endothelial nitric oxide synthase（eNOS） gene with the decompression effects of candesartan in essential hypertensive patients. Methods 203 patients with essential hypertension were enrolled in and treated with candesartan for 4 weeks. The polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method was used to detect genetypes in hypertensive patients. Results The frequency of G allele was 85.71% and T allele was 14.29%. Before treatment, SBP, DBP in patients with T allele of eNOS gene G894T polymorphisrn （165±14.2） mmHg,（97±10.5）mmHg respectively, is not different with which of GG genotype （164±13.9）mmHg（96±11.2）mmHg;After 4 weeks＇treatment of candesartan, the decrease of SBP, DBP with GGgenotype patients was （13：6±6.6） mmHg, （11.4±4.3）mmHg respectively, which were higher than that of T allele genotype （9.2±5.7）mmHg, （8.5±4.4）mmHg, there is statistically significant diferrence（ P〈0.05）.Conclusion There is the association of eNOS gene G894T polymorphisms with decompression effects of candesartan in Dongxiang minority essential hypertensive patients.

关 键 词：高血压 一氧化氮合酶 基因多态性 降压疗效 

分 类 号：R544.1[医药卫生—心血管疾病] R972.4[医药卫生—内科学]