促卵泡生成素、黄体生成素和胰岛素对多囊卵巢病人颗粒细胞甾体激素分泌的影响  

The effects of FSH,LH and insulin on steroids production by granulosa cells from polycystic ovaries syndrome

作　　者：王蔼明[1,2] 卢春华[1,2] 乔杰[1,2] 李美芝[1,2] 

机构地区：[1]北京医科大学第三医院妇产科 [2]中国人民解放军海军总医院妇产科

出　　处：《中华医学杂志》1998年第11期830-832,共3页National Medical Journal of China

基　　金：卫生部科研基金

摘　　要：目的研究在促卵泡生成素（ＦＳＨ）、促黄体生成素（ＬＨ）和胰岛素（ＩＮＳ）刺激作用下多囊卵巢综合症（ＰＣＯＳ）病人与正常卵巢的颗粒细胞甾体激素产生的异同。方法对１８例月经周期正常妇女卵泡期的３３个卵泡和８例ＰＣＯＳ的１００个卵泡的颗粒细胞行体外培养，利用放射免疫法测定培养液中的甾体激素水平，比较了两组颗粒细胞在ＦＳＨ、ＬＨ和ＩＮＳ刺激作用下雌二醇（Ｅ２）和孕酮（Ｐ）的产生。结果ＰＣＯＳ病人的颗粒细胞在基础状态下，ＦＳＨ和ＩＮＳ刺激作用下Ｅ２产生量明显高于对照组（Ｐ＜００５～００１），在ＦＳＨ、ＬＨ和ＩＮＳ刺激作用下ＰＣＯＳ组产生的Ｐ量明显高于对照组（Ｐ＜００５～００１）；ＩＮＳ能明显的增强ＦＳＨ和ＬＨ对颗粒细胞的刺激作用；外周胰岛素拮抗的ＰＣＯＳ病人的颗粒细胞对ＩＮＳ刺激仍然敏感。结论ＰＣＯＳ病人的颗粒细胞甾体激素合成酶在体内时可能已被活化。Objectives To investigate steroids production of granulosa cells obtained from normal ovaries and polycystic ovaries from polycystic ovary syndrome (PCOS) patients under FSH, LH and insulin stimulation. Methods Granulosa cells of 33 folicles from 18 pairs of normal ovaries and about 100 folicles from 8 pairs of PCOS were cultured. Estrodial (E 2) and progesterone (P) accumulation in the medium was determined under different incubated conditions by RIA. Results Granulosa cells from PCOS produced more E 2 ( P <0.05～0.01) under basal, FSH and insulin stimulation and more P ( P <0.05～0.01) under FSH, LH and insulin stimulation than controls. Insulin markedly augmented FSH and LH stimulated steroid accumulation by two groups of granulosa cells. Granulosa cells from PCOS with peripheral insulin resistance also responded to insulin. Conclusion Enzymes for steroids synthesis in granulosa cells from PCOS may be activated in vivo, and hyperinsulinisma and elevated LH levels in PCOS may be contributable.

关 键 词：多囊卵巢综合症 激素 高胰岛素血症 FSH LH INS 

分 类 号：R711.75[医药卫生—妇产科学]