引导RNase P对HCMV UL54 mRNA片段体外切割的EGS构建  

Construction of EGS for directing targeted cleavage of human cytomegalovirus UL54 mRNA segments with RNase P

作　　者：吕静竹[1,2] 李月琴[2] 周天鸿[2] 

机构地区：[1]安徽理工大学医学院,淮南232001 [2]暨南大学生命科学技术学院,广州510632

出　　处：《生物学杂志》2009年第5期4-6,共3页Journal of Biology

基　　金：国家自然科学基金(No.30370776);广东省自然科学基金重大项目(No.36703);广东省自然科学基金(No.021162;000718)

摘　　要：HCMV是一种广泛存在的疱疹病毒,在免疫抑制和免疫功能低下人群中,HCMV感染可引起严重疾病。RNase P是细胞内催化tRNA5′末端成熟的酶,当EGSs与靶mRNA互补结合并形成类似tNRA的复合物时,大肠杆菌RNase P催化亚基M1 RNA可具备对靶mRNA特异的催化切割活性。为研究抗病毒制剂,针对HCMV DNA多聚酶UL54 mRNA设计并构建特异性的EGS-C6,通过对UL54基因亚克隆片段转录产物体外切割研究,证实该EGS具备引导M1 RNA对UL54 mRNA特异切割的能力,可发展成一种新型抗病毒制剂。HCMV is a ubiquitous herpesvirus. Infections by this virus can cause severe health problems in immunosuppressed and immunoeompromised individuals. RNase P is an enzyme which catalyzes a hydrolysis reaction to remove the leader sequence of precursor tRNA. When external guide sequences hybridize to the mRNA to form a structure resembling a tRNA substrate, M1 RNA from Escherichia coli can cleave the target mRNA specifically. To research antiviral agents, a sequence-specific EGS-C6 was used to target UL54 mRNA encoding major DNA polymerase of human cytomegalovirus. The results showed that EGS-C6 could direct the specific cleavage of UL54 mRNA in vitro and could be developed as a novel antiviral agent.

关 键 词：RNASE P 外部引导序列 人巨细胞病毒 UL54基因 

分 类 号：Q783.1[生物学—分子生物学]