发光金纳米粒子测定曲通X-100及其临界胶束浓度  

Determination of Triton X-100 and Its Critical Micelle Concentration with Luminescence Gold Nanoparticles

作　　者：刘玫瑰[1] 曹春[1] 曹明[1] 朱昌青[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,安徽省化学与生物传感重点实验室,芜湖241000

出　　处：《分析化学》2009年第10期1503-1506,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20575002);安徽省自然科学基金(No.070416239)资助项目

摘　　要：参照文献方法合成了BSA保护的水溶性发光金纳米粒子,并考察了此探针在非离子表面活性剂曲通X-100中的发光行为。根据观察到的发光增强效应,建立了一种简单的测定曲通X-100的方法。考察了发光金纳米粒子的浓度、体系酸度、反应时间及共存物质对测定的影响。在最佳条件下,发光强度与曲通X-100的浓度分别在0～150μmol/L和150～600μmol/L范围内分段成正比关系。两条工作曲线的交点所对应的浓度与曲通X-100的临界胶束浓度十分吻合,为胶束形成过程提供了直接的指示。作为一种生物相容性探针,发光金纳米粒子被用于生物学样品中曲通X-100的分析测定,结果令人满意。Water-soluble BSA-protected gold nanoparticles were synthesized according to the literature,and the luminescence behavior of the probes in surfactant Triton X-100 was investigated.A facile method for the determination of Triton X-100 has been developed based on the enhancement effect of Triton X-100 on the luminescence of gold nanoparticles.The effects of concentration of luminescent gold nanoparticles,pH,reaction time,and coexisted materials on the determination have been investigated.Under the optimum conditions,the luminescence intensity was proportional to the Triton X-100 concentration in the range of 0-150 μmol/L and 150-600 μmol/L,respectively.The concentration corresponding to the point of intersection of the two calibration curves was in good agreement with the critical micelle concentration of surfactant Triton X-100,which gave a direct indication for the micelle forming process.As novel biocompatible fluorophores,luminescence gold nanoparticles have been applied to the determination of Triton X-100 in some biological samples with satisfactory results.

关 键 词：曲通X-100 临界胶束浓度 发光金纳米粒子 

分 类 号：O648.1[理学—物理化学]