人粒细胞集落刺激因子cDNA的克隆及其在CHO细胞的表达  

The Cloning and Expression of Human G-CSF in CHO

作　　者：王江方[1] 张学光[1] 刘培岭 张雁云[1] 顾宗江[1] HalletMM 

机构地区：[1]苏州医学院免疫学研究室,215007

出　　处：《苏州医学院学报》1998年第9期894-896,共3页Acta Academiae Medicinae Suzhou

基　　金：核工业总公司人造血生长因子(G-CSF)基因工程产品的研究和开发课题!H34162940301

摘　　要：该文以PKCR6质粒为载体，构建了人体细胞集落刺激因子（hG－CSF）的次级克隆PKCR6－G－CSF，并成功转染CHO细胞，获得高表达、稳定分泌人G－CSF的转基因细胞，为扩大规模基因工程生产重组细胞因子奠定了一定的基础。The secondary cloning vector PKCR6 carrying human G-CSF cDNA was constructed with PKCR6 plasmid.The CHO cell line Which highly expressed G-CSF gene was successfully obtained, and the stability of cloned CHO supernant is stable. Therefore, this study can be the fundament of producing protein with gene cloning method in large scale for clinical application.

关 键 词：CHO细胞 hG-CSF CDNA 克隆 PKCR6质粒 

分 类 号：R392.2[医药卫生—免疫学] R394[医药卫生—基础医学]