结核杆菌特异性抗原对结核病诊断价值研究  

Value of detection of ESAT-6,CFP10 and 38KD of Mycobacterium tuberculosis antigen in diagnosis of tuberculosis

作　　者：王桥霞[1] 滕林[2] 曲延文[1] 

机构地区：[1]山东省胜利石油管理局胜利医院,山东东营257055 [2]潍坊市人民医院,山东潍坊261000

出　　处：《中国热带医学》2009年第11期2085-2086,共2页China Tropical Medicine

摘　　要：目的从人血清提取结核杆菌早期分泌性抗原靶6、培养滤液蛋白、结核杆菌分泌蛋白38KD进行标测，探讨结核病诊断新方法。方法选择我院2007年1月～2008年1月间住院肺结核病人和健康查体者，应用结核分枝杆菌相关性抗原ESAT-6、CFP10、38KD检测试剂盒测定血清结核菌抗原水平，同时对所有入选者均行结核菌培养。结果活动性肺结核患者血清中检测到结核分枝杆菌特异性抗原ESAT-6、CFP10、38KD的阳性率高，与健康对照组有统计学差异（P〈0．005）；结核分枝杆菌抗原可区分结核感染和非感染，与结核菌素试验比较有显著的统计学差异（P〈0．001）。结论血清结核杆菌特异性抗原能够作为诊断结核病的依据，对鉴别活动性肺结核、非活动性肺结核、非结核病具有重要的价值。Objective To seek new methods for detection of Mycobacterium tuberculosis by detection of ESAT-6, CFP10 and 38KD with ELISA. Methods The antigen of ESAT-6,CFP10 and 38KD secreted by Mycobacterium tuberculosis in 398 patients and 200 healthy controls were detected from January 2007 to January 2008 by ELISA doubleantibody sandwich method and Mycobacterium tuberculosis was cultured in all the selected simultaneously. Results 1. ESAT-6,CFP10,and 38KD secreted by Myeobacterium tuberculosis were detected in the active pulmonary tuberculosis patients and Mycobacterium tuberculosis specific antigen was not detected in the healthy controls （P〈0.005）.2. Mycobacterium tuberculosis specific antigen can distinguish tuberculosis infection from non- tuberculosis infection; showing significant difference comparing with tuberculin testing in statistics （P〈0.001）. Conclusion Mycobacterium tuberculosis specific antigen can distinguish tuberculosis infection from non- tuberculosis and it is of important value in diagnosis of Mmycobacterium tuberculosis infection.

关 键 词：肺结核 结核杆菌 相关性抗原 早期分泌抗原靶6 培养滤液蛋白10 

分 类 号：R542.1[医药卫生—心血管疾病]