头孢拉定流动注射-化学发光分析法的研究  

Investigation on Flow Injection-Chemiluminescence Method for Determination of Cefradine

作　　者：陈效兰[1] 肖柳婧 徐淑静[1] 杨娟[1] 

机构地区：[1]湘潭大学化学学院环境友好化学与应用省部共建教育部重点实验室,湖南湘潭411105

出　　处：《湘潭大学自然科学学报》2009年第3期81-84,共4页Natural Science Journal of Xiangtan University

摘　　要：在氢氧化钠反应介质中，头孢拉定对鲁米诺-H2O2体系产生的化学发光有显著增强作用，结合流动注射技术，建立了一种简单，灵敏，稳定的流动注射化学发光测定头孢拉定的新方法．研究结果表明，在0．10mol／L氢氧化钠介质中，5.0×10^-4mol／L鲁米诺与5．0×10—3mol／LH2O2反应，头孢拉定对此化学发光具有显著增强作用．该方法的线性范围为1．0×10^-8～1．0×10^-6g／L，检出限为6．1×10^-11g／L，对1．0×10^-8g／L的头孢拉定连续平行测定11次，方法的相对标准偏差（RSD）为1．9％．该法已用于头孢拉定胶囊中头孢拉定含量的测定，回收率在98．8-103．2％之间．A novel ehemiluminescenee（CL） method coupled with flow-injection（F1） technique for the determination of cefradine was developed. It was based on the fact that cefradine could enhance chemiluminescence intensity from the reaction of Luminol with H2O2 in sodium hydroxide medium. The optimum experimental conditions include the use of 0. 10 mol/L NaOH, 5.0 × 10^-4 mol/L luminol and 5.0 × 10^-3 mol/L H2O2. The concentration of cefradine showed good linear relationship in the range of 1.0× 10^-8- 1.0 × 10^-8 g/L with a detection limit of 6.1 × 10^-11 g/L, and the relative standard deviation（RSD） was 1.9% （ n = 11, ρ -1.0×10^-8 g/L）. This method was applied to determine cefradine in the capsules, and the recoveries were between 98.80%-103.2%.

关 键 词：流动注射 化学发光 鲁米诺 H2O2 头孢拉定 

分 类 号：O657.3[理学—分析化学]