大鼠亚急性吸入高效氯氰菊酯的毒性研究  

Study on the subacute inhalation toxicity of beta-cypermethrin in SD rats

作　　者：黄振烈[1] 陈润涛[1] 宋向荣[1] 阮小林[1] 梁丽燕[1] 

机构地区：[1]广东省职业病防治院,广东广州510300

出　　处：《中国工业医学杂志》2009年第5期340-343,F0003,共5页Chinese Journal of Industrial Medicine

摘　　要：目的研究高效氯氰菊酯原药的亚急性吸入毒性,为确定亚急性最大无作用浓度及靶器官提供依据。方法按GB15670-1995《农药登记毒理学试验方法》进行。SPF级SD大鼠,设5个组,分别为空白对照组、溶剂对照组及受试物低、中、高浓度组,每组12只,雌雄各半。空白和溶剂对照组分别喷入空气和溶剂二甲基亚砜;低、中、高浓度组分别用不同浓度(3.36、16.61、111.05 mg/m3)的受试物行动物吸入染毒28 d。试验结束时,分别对动物作血液常规、生化、体重和脏器系数等测定,并进行组织病理学检查。结果高浓度组受试动物体重有明显降低,脑体比升高,部分动物坐骨神经纤维内轴突肿胀,有轻微的脱髓鞘改变;雌性动物淋巴细胞(LY)计数百分率降低,肾上腺体比、红细胞平均体积(MCV)和平均血红蛋白量(MCH)升高;雄性动物肾体比、睾丸体比和附睾体比升高,总精子数和平均血红蛋白浓度(MCHC)降低。中、高浓度组雄性动物血清尿素氮(BUN)升高,精子活力降低。结论高效氯氰菊酯原药在SD大鼠亚急性(28 d)吸入毒性试验的最大无作用浓度为3.36 mg/m3(4 h/d),在111.05 mg/m3(4 h/d)的染毒条件下有一定的神经毒性和雄性生殖毒性。Objective To explore the subacute toxicity of beta-cypermethrin （β-CP） by subacute inhalation in SD rats, thereby provide the evidences for determinating its maximum ineffective concentration and target. Methods SD rats were randomly divided into 5 groups, they were control group, solvent control group and 3 exposed groups （there were 6 female rats and 6 male rats for each group）. The exposed dose of β-CP for these groups were 0, 0, 3.36, 16. 61, 111.05 mg/m^3 , respectively ; 4 hours per day for 28 days. At the end of experiment, the rats were killed, the routine and biochemical detection, the body-weight and organ coefficient were all measured. Results In the high exposure group, growth of body weight was significantly inhibited, brain weight/body weight ratio was risen; axonal swelling and mild demyelination in sciatic nerve were observed in some rats ; in female rats, lymphocyte percentages in blood decreased, MCV, MCH and adrenal gland to body weight ratio were increased; in male rats, the kidney, testis and epididymis weights to body weight ratios were all increased; while MCHC and total amounts of sperm were decreased. In both medium and high exposure groups, BUN levels of male rats were increased, sperm vitalities were decreased. Conclusions The results suggested that the maximum ineffective concentration of β-CP in SD rats was 3.36 mg/m^3 （4 h/d）, and there were some neurotoxicity and reproductive toxicity at the concentration of 111.05 mg/ m^3 （4 h/d）.

关 键 词：高效氯氰菊酯 亚急性吸入毒性 最大无作用浓度 神经毒性 生殖毒性 

分 类 号：R996[医药卫生—毒理学]