薄层扫描法测定首清颗粒中黄芪甲苷的含量  

Determination of Astragaloside Ⅳ in Shouqing Granula by TLC-Scanning

作　　者：侯凤飞[1] 张海鸣[2] 曹杰[2] 沈圣民[2] 杜树山[2] 

机构地区：[1]中国中医药科技开发交流中心,北京100028 [2]北京师范大学资源学院教育部资源药物工程研究中心,北京100875

出　　处：《中国中医药信息杂志》2009年第11期44-45,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家科技支撑计划(2007BA148B10);北京市新医药学科群重点支持项目(xk100270569)

摘　　要：目的建立首清颗粒中黄芪甲苷含量的测定方法。方法采用薄层扫描法,硅胶G薄层预制板,氯仿-甲醇-水(13:6:2)10℃以下放置过夜的下层溶液为展开剂,测定波长530 nm,参比波长700 nm。结果黄芪甲苷点样量在0.22～0.73 μg之间与吸收度积分值呈良好的线性关系,平均回收率为97.72%,RSD =0.69%。结论该法便捷、灵敏、准确,可作为首清颗粒的质量控制方法。Objective To estalish an accurate method for determining the content of astragaloside IV in Shouqing Granula. Method TLC scanner was selected to detect astragaloside IV with silica gela thin layer. The sample was separated by using chloroform-methanol-water （13 ： 6 ： 2） below 10 ℃ overnight with the under layer solution, λ s=530 nm, λ R=700 rim. Results The linear ranges of astragaloside IV was 0.22- 0.73 μg, the average recovery was 97.72%, RSD=0.69%. Conclusion The method was simple and accurate, and can be used for the quality control of Shouqing Granula.

关 键 词：首清颗粒 黄芪甲苷 薄层扫描法 含量测定 

分 类 号：R284.1[医药卫生—中药学]