HPLC-ELSD法测定轻身减肥片中黄芪甲苷的含量  

Content Determination of Astragaloside Ⅳ in Qingshen Jianfei Tablets by HPLC-ELSD

作　　者：李战 李美容 罗国琼 阮群 

机构地区：[1]广西玉林制药有限责任公司,广西玉林537001

出　　处：《中国中医药信息杂志》2009年第11期46-47,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立HPLC-ELSD测定轻身减肥片中黄芪甲苷含量的方法。方法选用C_(18)色谱柱(4.6mm×250mm,5μm),流动相为乙腈-水(35:65)蒸发温度120℃,雾化温度80℃,载气流速1.6 mL/min,柱温35℃。结果黄芪甲苷在1.03～8.24 μg范围内呈良好的线性关系,r = 0.999 9,平均加样回收率为99.35%,RSD = 1.36% (n = 6)。结论所建立的方法简便、准确,重复性好,精密度高,可用于轻身减肥片的质量控制。Objective To establish a HPLC-ELSD method for determining the content of astragaloside Ⅳ in Qingshen dianfei Tablets. Methods Stationary phase was C18 column [4.6 mm × 250 mm, 5μm）, mobile phase was acetonitrice-water （35 ： 65）. The evaporation temperature was 120 ℃, the pulverization temperature was 80 ℃, the flow rate was 1.6 mL/min and column temperature was 35 ℃. Results The standard curve was linear within the range of 1.03-8.24 μg, r =0.9999. The average recovery was 99.35% with RSD = 1.36% （n = 6）. Conclusion The established method is simple and accurate, with good reproducibility and high precision, and suitable for the determination of astragaloside Ⅳ in Qingshen dianfei Tablets.

关 键 词：轻身减肥片 黄芪甲苷 高效液相色谱-蒸发光散射检测器 含量测定 

分 类 号：R284.1[医药卫生—中药学]