内障丸对青春期PCOS大鼠卵巢颗粒细胞凋亡因子Bcl-2及Bax的影响  

Effect of Rou Zhang Pills on Apoptosis Factor Bcl-2 and Bax of Ovarian Granulosa Cells in Adolescent PCOS Rats

作　　者：姚美玉[1] 李祥 陈皇珍[3] 

机构地区：[1]黑龙江中医药大学附属第一医院妇科,黑龙江哈儿滨150040 [2]黑龙江省五常市中医院,黑龙江五常150200 [3]黑龙江中医药大学

出　　处：《实用中医药杂志》2009年第11期716-717,共2页Journal of Practical Traditional Chinese Medicine

摘　　要：目的:探讨内障丸对青春期PCOS大鼠卵巢颗粒细胞凋亡因子Bcl-2及Bax表达的影响。方法:以23日龄幼年SD清洁雌性大鼠为研究对象,应用皮下埋植左炔诺孕酮硅胶棒联合皮下注射HCG法建立青春期PCOS动物模型,运用免疫组织化学SABC方法及光、电镜技术等方法,检测大鼠造模前后及给药前后卵巢颗粒细胞Bcl-2,Bax的表达情况。结果:造模后模型组颗粒细胞Bcl-2,Bax,Bcl-2/Bax表达水平与正常对照组差异明显,运用阳性目标面密度进行比较,经治疗后内障丸高、中、低剂量组Bcl-2表达均强于Bax。其中高剂量组的表达与正常组接近。在对照组中,Bax表达较Bcl-2强。结论:内障丸可以抑制多囊卵巢大鼠卵巢颗粒细胞凋亡及卵泡闭锁,而且可以使多囊卵巢大鼠卵泡闭锁时间提前,减轻卵巢多囊样改变,从而改善卵巢功能。Objective：To investigate the influence onovary granular cell apoptosis factor Bcl -2 and Bax ofpuberty PCO rats treated with Neizhang Pill, Methods ： Take the 23 - day - old immature female SD rats as the object of study, progesterone - synchronized immature rats were used as the animal model for PCO by in -jecting HCG. Compared with normal control group,investigate the changement of the modelgroup＇s ovary granular cell apoptosis factor Bcl -2 and Bax. Results：The treatment group＇s expressions of Bcl -2 and Bax not differ from the control group＇s. Conclusions：Neizhang Pill could promote ovulation by inhibit ovary granular cell apoptosis and follicle atresia.

关 键 词：内障丸 大鼠 青春期PCOS 细胞凋亡 BCL-2 BAX 影响 

分 类 号：R285.5[医药卫生—中药学]