低硒与Coxsackie B_(3m)病毒感染对BALB/c小鼠肝脏脂质过氧化物代谢的影响  

Effect of selenium deficient diet and Coxsackie B 3m infection on activity of LPO in liver in BALB/c mice

作　　者：刘红[1] 刘艺[1] 殷秀云[1] 修英霞[1] 张邻杰[1] 周令望[1] 高彦辉[1] 钟学宽[1] 

机构地区：[1]哈尔滨医科大学克山病研究所

出　　处：《中国地方病学杂志》1998年第6期370-372,共3页Chinese Jouranl of Endemiology

基　　金：卫生部自然科学基金

摘　　要：用国产低硒食用酵母合成低硒饲料（硒０．０１３ｍｇ／ｋｇ，ＶＥ含量为２０ｍｇ／１００ｇ）喂养断奶后ＢＡＬＢ／Ｃ雄性小鼠，５周后腹腔接种嗜鼠心肌病毒ＣＶＢ３ｍ１０３ＴＣＤ５００．１ｍｌ，７天后处死建立低硒状态下病毒性心肌炎模型，测定肝脏组织中脂质过氧化物（ＬＰＯ）含量及全血中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性。结果表明低硒感染ＣＶＢ３ｍ小鼠肝组织中ＬＰＯ含量明显高于常硒病毒感染组及常硒对照组（Ｐ＜０．０１）；低硒感染ＣＶＢ３ｍ病毒组小鼠全血ＧＳＨ－Ｐｘ活力也最低。结果提示：低硒因素加重病毒感染引起ＧＳＨ－Ｐｘ活力降低，ＬＰＯ堆积。A virus myocarditis model had been established in young male BALB/c mice in which milk feeding just had been ceased.In this model,BALB/c mice had been fed with selenium (Se) deficient diet made in Se deficient yeast (Se:0.013ppm;VE:20mg/100g) for 5 weeks,and then inoculated with myocardiopathic Coxsackie B type 3m (CVB 3m ),10 3 TCD 50 0.1ml intraperitoneally.The seventh day after viral infection,when myocarditis in low Se BALB/c mice was established,the concentration of LPO in livers and activity of sera glutathione peroxidase (GSH Px) were measured.Our results demonstrated that the concentration of LPO in livers was significantly higher and activity of sera GSH Px was significantly lower in Se deficient infected mice than those in infected mice fed with normal diet.These suggest that lower Se intake may worsen the lowered activity of GSH Px and accummulation of LPO in livers caused by CVB 3m viruses,which may reduce the antioxidation activity in infected mice.

关 键 词：低硒 CVB3m病毒 心肌炎 脂质过氧化物 GSH-PX 

分 类 号：R542.21[医药卫生—心血管疾病] R373.23[医药卫生—内科学]