TRITURUS变色龙与雅培I2000两种仪器检测乙肝特殊模式分析  

Hepatitis B Detecting Results Comparison between TRITURUS and Abbott I2000

作　　者：田树萍[1] 于艳华[1] 辛咏梅[1] 孙桂珍[1] 

机构地区：[1]首都医科大学附属北京佑安医院临床检验中心,北京100069

出　　处：《中国医疗设备》2009年第10期87-88,共2页China Medical Devices

摘　　要：目的探讨酶联免疫吸附试验(ELISA)代表机型TRITURUS变色龙、微粒子免疫化学发光分析仪(MEIA)代表机型雅培I2000两种仪器方法检测乙型肝炎特殊模式分析。方法用ELISA法筛选出特殊模式用MEIA法再次检测,并进行HBV-DNA含量检测,对其结果进行对比分析。结果ELISA与MEIA两种仪器及检测方法对特殊模式的检出率比较差异有统计学意义(P<0.05);HBeAg与HBV-DNA定量检测有显著统计学意义(P<0.01)。结论MEIA定量检测乙型肝炎特殊模式灵敏度、速度和自动化程度均较高,是目前临床检查乙肝"两对半"的较好方法,特殊模式检出明显高于ELISA试验。在实际工作中ELISA法检测特殊模式时应联合HBV-DNA含量检测结果共同分析,有利于乙型肝炎的临床诊断、疗效观察和预后判断。Objective To compare hepatitis B detecting results between TRITURUS and Abbott I2000.Methods By enzyme-linked immunosorbent assay （ELISA） screening of a special model with chemiluminescent microparticle immunoassay （MEIA） re-testing, and detection of HBV-DNA content, the results were analyzed.Results The detection rate comparison between ELISA and MEIA was significant （P〈0.05）, HBeAg and HBV- DNA quantitative detection results were significant. Conclusion MEIA is the better method of detecion of hepatitis B,while ELISA detection combining with HBV-DNA quantitative detection is propitious to the diagnosis, curative effect observation and prognosis judgement.

关 键 词：酶联免疫分析仪 微粒子免疫化学发光分析仪 乙肝特殊模式 乙肝“两对半” 

分 类 号：TH776[机械工程—仪器科学与技术] R512.62[机械工程—精密仪器及机械]