山楂总三萜酸对大鼠肝细胞合成^(14)C-胆固醇及肝细胞膜HDL受体活性的影响  

Effect of Shanzha total triterpenoid acid on holesterol synthesis and activity of HDL receptor in hepatocyte of rat

作　　者：马路[1] 史大卓[2] 陈可冀[2] 刘剑刚[2] 

机构地区：[1]中国人民解放军海军总医院,北京100037 [2]中国中医科学院西苑医院,北京100091

出　　处：《中国医院药学杂志》2009年第21期1807-1810,共4页Chinese Journal of Hospital Pharmacy

基　　金：中国博士后基金资助项目(编号:2004035408)

摘　　要：目的:探讨山楂降血脂有效部位-山楂总三萜酸的作用机制。方法:采用体外大鼠肝细胞培养,以液体闪烁计数法测定肝细胞中合成14C-胆固醇的量、生物素-亲和素酶联(BAE)法测定肝细胞膜高密度蛋白(HDL)受体活性。结果:山楂总三萜酸对14C-胆固醇的合成有一定的阻抑作用;用药组与正常对照比较,肝细胞膜HDL受体亲和常数(Kd)无明显变化,最大特异性结合量(Bmax)显著增加(P<0.05)。表明山楂总三萜酸可使大鼠肝细胞膜HDL受体呈现以受体数目增加为特征的活性升高。结论:山楂总三萜酸降血脂作用可能与其提高HDL受体的活性、抑制胆固醇合成有关。OBJECTIVE To study the mechanism of total triterpenoid acid of Crataegus pinnatifida in decreasing blood-lipid. METHODS Primary cultures of rat hepatocytes in vitro were used to assay the cholesterol synthesis of 14C-acetate by liquid scintillation count; Activity of rat hepatic high-density lipoprotein（HDL）receptor was measured with biotin-avidion ELISA （BAE）. RESULTS Triterpenoid acid of Crataegus pinnatifida inhibited cholesterol synthesis of 14C-acetate and enhanced activity of HDL receptor. CONCLUSION The effect of triterpenoid acid of Crataegus pinnatifida on decreasing blood-lipid may be related to inhibiting of cholesterol synthesis and enhancing activity of HDL receptor.

关 键 词：山楂 总三萜酸 肝细胞培养 高密度脂蛋白受体 

分 类 号：R285.5[医药卫生—中药学]