血小板相关抗体对巨核细胞增殖的实验研究  

The Effect of Platelet-Associated Antibody on Megakaryocyte Proliferation

作　　者：汤喜兰[1] 黄立新[2] 曾治君[1] 张启云[1] 单义民[1] 徐国良[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004 [2]江西艺术职业学院基础部,南昌330013

出　　处：《江西中医学院学报》2009年第5期58-60,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：江西省自然科学基金资助项目(0640161)

摘　　要：目的:探讨血小板相关抗体(platelet-associated antibody,PAIgG)对巨核细胞体外增殖的影响。方法:制备骨髓成纤维细胞无血清条件培养液,采用小鼠骨髓巨核细胞原代培养的方法,探讨PAIgG对巨核细胞体外增殖的影响。结果:50倍和100倍稀释的PAIgG能够显著抑制巨核细胞的增殖。结论:PAIgG对巨核细胞的体外增殖有抑制作用。Objective：To investigate the effect of platelet-associated antibody （PAIgG） on the expansion of mature megakaryocytes in vitro. Methods：Serum-free bone marrow fibroblasts conditioned medium（F-CM） was prepared and collected. The method of bone marrow megakaryocytes primary culture was used and The effect of PAIgG on the expansion of megakaryocytes was discussed. Results： PAIgG that diluted for 50 times and 100 times can significantly inhibit megakaryocytes proliferation. Conclusion ： PAIgG can successfully inhibit megakaryocytes expansion in vitro.

关 键 词：血小板相关抗体 巨核细胞 增殖 

分 类 号：R285.5[医药卫生—中药学]