O_3与CO_2浓度倍增对油松针叶抗氧化酶活性的影响  

Effects of elevated O_3 and CO_2 on the antioxidant enzyme activities in Pin us tabulaeformis needles.

作　　者：李响[1,2] 何兴元[1] 陈玮[1] 徐胜 颜坤[1,2] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016 [2]中国科学院研究生院,北京100039

出　　处：《生态学杂志》2009年第11期2220-2226,共7页Chinese Journal of Ecology

基　　金：国家自然科学基金重大研究计划重点项目(90411019);中国科学院知识创新工程重要方向资助项目(KZCX3-SW-436)

摘　　要：以4年生的油松幼苗为试材,采用开顶箱内气体熏蒸实验,对经高浓度O3(80nmol.mol-1)和CO2(700μmol.mol-1)单一及复合处理3年后,油松当年生针叶内丙二醛(MDA)含量、活性氧自由基和抗氧化酶等逆境生理指标进行了分析。结果表明:超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)和脱氢抗坏血酸还原酶(DHAR)活性经O3处理后显著升高,分别比对照上升了43.1%、18.9%、283.5%和142.6%,而CO2和复合处理仅对APX和CAT活性有明显的诱导作用,但均低于O3处理;O3处理导致MDA含量上升51.8%,而外加CO2则能有效逆转这一趋势;超氧阴离子(O2.-)产生速率和H2O2含量在所有处理组合间均无显著差异。上述结果说明,O3能有效地激活油松当年生针叶内的抗氧化酶系统,而CO2能缓解O3所带来的不利影响,但并非通过提高抗氧化酶活性这一途径。Four-year-old Pinus tabulaeformis seedlings were planted in open-topped chambers and treated with elevated O3 （ 80 nmol ·mol^ - 1 ） and/or CO2 （700 μmol · mol^ - 1 ）. After treated for three years, the malondialdehyde （MDA） content, reactive oxygen species, and antioxidant enzyme activities in the current year needles were analyzed. In treatment elevated O3 , the activi- ties of superoxide dismutase （SOD）, ascorbate peroxidase （APX）, catalase （CAT） and dehydroascorbate reductase （DHAR） increased significantly, being 43.1%, 18.9%, 283. 5% and 142.6% higher than the control; and in treatments elevated CO2 and its combination with elevated O3, only APX and CAT activities were induced remarkably but still lower than those in treatment elevated O3. Elevated O3 increased the MDA content by 51.8%, whereas the combination of elevated O3 and CO2 reversed this increase efficiently. No significant differences were observed in the superoxide anion （ O2^.- ） generating rate and H2O2 content among all treatments. All the results demonstrated that elevated O3 was able to effectively activate the antioxidant enzyme system in P. tabulaeformis current year needles, while elevated CO2 could mitigate the negative effects of elevated O3 through the actions other than enhancing the antioxidant enzyme activities.

关 键 词：高浓度O3 高浓度CO2 油松 抗氧化系统 

分 类 号：S791.254[农业科学—林木遗传育种]