传染性支气管炎病毒结构蛋白免疫鸡对CD4^+ CD8^+ T淋巴细胞亚群的影响  

The effects of immunizing chickens with IBV structural protein on CD4^+ and CD8^+ T lymphocytes

作　　者：汪雪[1,2] 王红宁[1] 阳泰[1] 鲁丹[2] 张云飞[2] 唐俊妮[1] 

机构地区：[1]四川大学生命科学学院动物疫病防控与食品安全四川省重点实验室,四川成都610064 [2]四川农业大学动物医学院,四川雅安625014

出　　处：《中国兽医杂志》2009年第10期13-15,共3页Chinese Journal of Veterinary Medicine

基　　金：国家"863"计划项目(2006AA10A205)

摘　　要：为了比较传染性支气管炎病毒3种主要结构蛋白免疫鸡后对CD4+、CD8+T淋巴细胞亚群的不同影响。本试验以pVAX1载体为携带工具,制备分别含有IBV主要结构蛋白基因的质粒免疫健康雏鸡,采用流式细胞仪(FACS)对免疫鸡外周血中CD4+、CD8+T淋巴细胞数进行检测。结果显示:各试验组间,携带N蛋白基因的质粒在免疫后3周内CD4+T淋巴细胞数和CD8+T淋巴细胞数均高于其他组,且差异极显著(P<0.01)。由此可见,IBV的N蛋白可明显诱导CD8+T淋巴细胞的CTL免疫作用和CD4+T淋巴细胞的辅助性免疫作用,其细胞免疫原性高于S1蛋白和M蛋白。Objective. To study the effects of immunizing chickens with IBV structural protein on CD4^＋ and CD8^＋ T lymphocytes. Methods. The eukaryotic expression vector pVAX1 was used to express the IBV main strutural protein in chicken. Fluorescence activated cell sorter （FACS） methods were used to detect the number of CD4^＋ and CD8^＋ T lymphocytes. Results, The number of CD4^＋ T cells induced by N protein 7 days after inoculation was the highest of all the groups, and appeared significant different as compared to the other group at 1-3 weeks （P〈0.01）. CD8^＋ T lymphocytes also demonstrated the same change. Conclusion. The N protein of IBV could induce the higher number of CD8^＋（CTL） and CD4^＋ （TH） than M and S1 protein.

关 键 词：禽传染性支气管炎 结构蛋白 CD4+ CD8+ 

分 类 号：S852.65[农业科学—基础兽医学] S852.4[农业科学—兽医学]