丙泊酚静脉输注对老年大鼠海马内M受体和NMDA受体的影响  

Effect of intravenous propofol on muscarinic or NMDA receptor in the brain of aged rats

作　　者：刘秀芬[1] 吴新民[1] 刘毓和[1] 左萍萍[2] 杨楠[2] 

机构地区：[1]北京大学第一医院麻醉科,北京100034 [2]中国医学科学院基础医学研究所药理室,北京100005

出　　处：《中国新药杂志》2009年第20期1975-1978,1982,共5页Chinese Journal of New Drugs

摘　　要：目的:观察静脉持续输注丙泊酚(P)于给药后不同时间对老年大鼠大脑海马组织中胆碱能毒蕈碱样受体(M-R)和N-甲基-D-天门冬氨酸受体(NMDA-R)的影响。方法:在18月龄雄性或雌性SD大鼠,尾静脉注射丙泊酚10mg·kg-1诱导麻醉,然后恒速输注25或45mg·kg-1·h-1维持2h或4h;于丙泊酚给药后1d(近期影响组)或6,12d(远期影响组)急性处死大鼠,分离脑组织海马,用放射配体结合分析法测定M-R与[3H]-QNB以及NMDA-R与[3H]-MK-801的特异性结合量。结果:近期影响组和远期影响组分别与其对照组比较,M-R与[3H]-QNB特异性结合量及NMDA-R与[3H]-MK-801特异性结合量均无统计学差异。结论:丙泊酚静脉给药对老年大鼠海马M-R与[3H]-QNB特异性结合量及NMDA-R与[3H]-MK-801特异性结合量无近期和远期影响。Objective：To investigate the transient and permanent effect of continuous intravenous infusion of propofol at different doses on muscarinic receptor （M-R） and N-methy-D-aspartate receptor （NMDA-R） in the brain of aged rats.Methods：In 18-months-old male or female SD rats,anesthesia was induced by intravenous injection of propofol （10 mg·kg^-1） through tail vein,and maintained by small or large dose （25 or 45 mg·kg^-1·h^-1） for different time length （2h or 4h）.Rats were decapitated and the hippocampus was removed 1d （for transient effect）,or 6d and 12d （for permanent effect） after administration.The binding capacities of M-R with [3H]-QNB and NMDA-R with [3H]-MK-801 were measured by radiant ligand binding analysis.Results：In transient and permanent effect groups,the binding capacities of M-R with [3H]-QNB and NMDA-R with [3H]-MK-801 were not significantly different as compared with corresponding control.Conclusion：Intravenous propofol has neither transient nor permanent effect on the binding capacities of M-R with [3H]-QNB and NMDA-R with [3H]-MK-801 in the hippocampus of aged rats.

关 键 词：丙泊酚 老年 受体 毒蕈碱 N-甲基-D-天门冬氨酸 

分 类 号：R971.2[医药卫生—药品]