成熟液中不同添加物对猪卵母细胞体外成熟及孤雌胚胎发育的影响  

Effect of Different Additive of Maturation Media on Maturation of Porcine Oocytes and Development of Parthenogenetic Embryoes

作　　者：张立苹[1] 王亚刚[2] 李莉[2] 刘西梅[2] 乔宪凤[2] 许生成[1] 

机构地区：[1]青海大学农牧学院,西宁810016 [2]湖北省农业科学院畜牧兽医研究所/动物胚胎工程与分子育种湖北省重点实验室,武汉430064

出　　处：《湖北农业科学》2009年第10期2502-2505,共4页Hubei Agricultural Sciences

基　　金：国家转基因动物新品种培育专项(2008ZX08006-002;2008ZX08006-003;2008ZX08010-003;2008ZX08011-004)

摘　　要：试验研究了在成熟液中分别添加0.1%PVA、10%FBS和不同浓度的EGF(20、30、40、50 ng·mL-1)对猪卵母细胞体外成熟的影响,以确立猪卵母细胞体外成熟的最佳条件。结果表明,在成熟液中添加0.1%PVA和10%FBS后,对猪卵母细胞的成熟率影响无显著差异(P>0.05),对猪卵母细胞孤雌激活后36 h的卵裂率和第6天的桑葚胚率无显著差异(P>0.05);添加不同浓度的EGF对猪卵母细胞体外成熟率无显著差异(P>0.05);但是添加30和40 ng·mL-1 EGF组在36 h的卵裂率要极显著地高于添加20和50ng·mL-1 EGF的两组(P<0.01);而分别添加30、40和50 ng·mL-1 EGF 3组处理在第6天的桑葚胚率无显著差异,且极显著地高于添加20 ng·mL-1 EGF组的(P<0.01)。所以,在成熟液中添加0.1%PVA可以替代10%FBS,尤以添加30 ng·mL-1 EGF组对猪卵母细胞的体外成熟效果较好。The effect of 0.1% PVA,10% FBS and different concentration of EGF in the maturation media on maturation of porcine oocytes was studied.The results showed that ： Addition of 0.1% PVA and 10% FBS in maturation medium had no significant effect on maturation rate,cleavage rate of 36^th h and 6^th day morula rate（P〉0.05）;Addition of different concen-tration of EGF in maturation medium had no significant effect on maturation rate（P〉0.05）,but addition of 30 and 40 ng·mL^-1 EGF had higher cleavage rate of 36^th h compared with that of 20 and 50 ng·mL^-1（P〈0.01）,addition of 30,40 and 50 ng·mL^-1 EGF in maturation medium had no significant effect on 6^th day morula rate（P〉0.05）,and it was significantly higher than 20 ng·mL^-1（P〈0.01）.In conclusion,addition of 0.1% PVA could instead of 10% FBS in maturation medium,addition of 30 ng·mL^-1 EGF was the best for maturation of porcine oocytes and development of parthenogenetic embryoes.

关 键 词：猪卵母细胞 体外成熟 孤雌胚胎 

分 类 号：S828.36[农业科学—畜牧学] S814.8[农业科学—畜牧兽医]