超声微泡造影剂介导血管生成素-1基因治疗下肢缺血  

Microbubble mediating Ang-1 gene therapy of lower limb ischemia by using ultrasound

作　　者：陶政[1] 徐三荣[1] 刘文新[2] 陈保定[3] 

机构地区：[1]江苏大学附属医院普外科,江苏镇江212001 [2]江苏大学附属医院放射科,江苏镇江212001 [3]江苏大学附属医院超声科,江苏镇江212001

出　　处：《南京医科大学学报（自然科学版）》2009年第11期1534-1537,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨用超声微泡造影剂介导血管生成素-1(Ang-1)基因治疗兔下肢缺血的可行性。方法:将15只新西兰大白兔左侧股动脉及其分支切断结扎后随机分成3组:A组采用局部注射pEGFP/Ang-1质粒与超声微泡造影剂的混合物并用超声照射;B组采用局部注射pEGFP/Ang-1质粒;C组注射生理盐水作为对照组。治疗4周后进行血管造影,了解新生血管情况,免疫组化方法检测Ang-1的蛋白表达。结果:在侧支循环建立、胞浆棕褐色程度、新生血管方面,A组较多,B组其次,C组较少。结论:用超声微泡介导的Ang-1基因转染,可促进缺血骨骼肌的血管新生和侧支循环建立,为下肢缺血性疾病的基因治疗提供了一种新的途径。Objective：To investigate the feasibility of microbubble mediating Ang-1 gene therapy of lower limb ischemia by using ultrasound. Methods：Fifteen rabbits with the left femoral artery ligation were randomly and averagely devidied into 3 groups：group A was locally intramuscular injected with the mixture of plasmid pEGFP/Ang-1 and microbubble and accepted ultrasound local radiation, group B was locally intramuscular injected with the plasmid pEGFP/Ang-1 ,and group C was injected with NS. Four weeks post-treatment,angiography was applied to evaluate the collateral circulation,and the expression of Ang-1 was evaluate by immunochemical staining. Results：In the matter of the collateral circulation, the degree of brown in cytoplasm, new vascular of the treatment region, group A was superior to the group B,the group B was superior to the group C. Conclusion：Microbubble mediating Ang-1 gene therapy by using ultrasound could promote the collateral circulation and new vessels,it may be a new methods for the treatment of limb ischemia diseases.

关 键 词：超声 微泡造影剂 血管生成素-1 基因治疗 下肢缺血 

分 类 号：R543.5[医药卫生—心血管疾病]